X-radiation-induced transformation in a C3H mouse embryo-derived cell line.
X-radiation-induced transformation in a C3H mouse embryo-derived cell line.
复制标题
X 射线诱导 C3H 小鼠胚胎来源细胞系的转化。
作者：
Margaret Terzaghi;J. Little
可重复的x射线诱导的致癌转化已在小鼠胚胎成纤维细胞细胞系中得到证实。从转化灶中提取的细胞注入同基因宿主后形成恶性肿瘤。当x射线剂量高达400拉德时，每个活细胞的转化体数量呈指数增长。在400至1500拉德的剂量下，指数生长培养物的转化频率保持在2.3 × 10（-3）不变。在x射线剂量高达300拉德的情况下，生存率几乎没有变化。剂量大于300拉德与生存率呈指数下降有关；生存曲线的Do为175 rad。转化频率随每个培养皿中活细胞数量的变化而变化。当细胞数从400个/100 mm培养皿增加到1000个活细胞时，转化频率下降约10倍。在此密度范围以下，变换频率变化不大。致死性辐照前细胞的存在与辐照细胞的转化增强或未辐照细胞培养的转化诱导无关。两性霉素B （Fungizone）在实验开始后2 - 3周内加入培养基中，可抑制转化体的出现。
Reproducible X-ray-induced oncogenic transformation has been demonstrated in an established cell line of mouse embryo fibroblasts. Cells derived from transformed foci formed malignant tumors when injected into syngeneic hosts. An exponential increase in the number of transformants per viable cell occurred with doses of up to 400 rads of X-radiation. The transformation frequency in exponentially growing cultures remained constant at 2.3 x 10(-3) following doses of 400 to 1500 rads. There was little change in survival following X-ray doses up to 300 rads. Doses greater than 300 rads were associated with an exponential decline in survival; the Do for the survival curve was 175 rads. Transformation frequency varied with changes in the number of viable cells seeded per dish. There was about a 10-fold decline in the transformation frequency when the number of cells was increased from 400 to 1000 viable cells/100-mm Petri dish. Below this density range there was little change in transformation frequency. The presence of lethally preir-radiated cells was not associated with an enhancement of transformation in irradiated cells or with the induction of transformation in unirradiated cell cultures. Amphotericin B (Fungizone) inhibited the appearance of transformants when added to the culture medium within 2 to 3 weeks after initiation of the experiment.