AuAg nanocages/graphdiyne for rapid elimination and detection of trace pathogenic bacteria.
AuAg nanocages/graphdiyne for rapid elimination and detection of trace pathogenic bacteria.
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DOI：
10.1016/j.jcis.2022.01.046
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发表时间：
2022-01
影响因子：
9.9
通讯作者：
Qiang Bai;Hongyang Luo;Shugao Shi;Shen Liu;Lina Wang;Fanglin Du;Zhugen Yang;Zhiling Zhu
中科院分区：
文献类型：
--
作者：
Qiang Bai;Hongyang Luo;Shugao Shi;Shen Liu;Lina Wang;Fanglin Du;Zhugen Yang;Zhiling Zhu
我们制备了一种生物相容的AuAg纳米笼/石墨二烯@聚乙二醇(AuAg/GDY@PEG)复合材料。将AuAg和GDY联合使用，获得协同增强的光热效应，将GDY和AuAg的抗菌作用用于联合抗感染治疗。研究了Au-Ag/GDY@聚乙二醇的体外抗菌活性，结果表明其具有良好的广谱抗菌活性，杀菌率为99.999%。基于AuAg/GDY@PEG的光热转化能力，成功建立了一种简便的病原菌光热免疫分析方法。夹心免疫反应在微孔板上进行，含有抗体的微孔板与被测细菌特异性结合，被测细菌再与表面有抗体的材料结合，信号是808 nm近红外光下的温度变化。最低检测限(LOD)为。鼠伤寒杆菌的检测值为103CFU/mL−1，范围为103-107CFU/mL−1。该方法准确、快速、成本低，可用于食品中致病菌的现场检测。
We prepared a biocompatible AuAg nanocages/graphdiyne @ polyethylene glycol (AuAg/GDY@PEG) composite. The combination of AuAg and GDY to obtain a synergistically enhanced photothermal effect, and the antibacterial effect of GDY and AuAg are used in combined anti-infective therapy. The in vitro antibacterial activity of AuAg/GDY@PEG was investigated, showing an impressive broad-spectrum antibacterial activity with the killing rate > 99.999%. Based on the photothermal conversion ability of AuAg/GDY@PEG, a simple photothermal immunoassay for pathogenic bacteria was successfully established. Sandwich immune response was performed on a microporous plate, the microplate containing the antibody binds specifically to the bacterium being tested, which then binds to the material with the antibody on its surface, and the signal was a change in temperature under 808 nm near-infrared light. The limit of detection (LOD) forS. typhimuriumdetection is 103CFU mL−1, with a range of 103–107CFU mL−1. This method is accurate, rapid and low-cost, which can be used for on-site detection of pathogenic bacteria in food.