Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation.
Protein phosphatase-2A is activated in pig brain following cardiac arrest and resuscitation.
复制标题
心脏骤停和复苏后猪脑中的蛋白磷酸酶 2A 被激活。
DOI：
10.1007/s11011-007-9074-1
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发表时间：
2008
影响因子：
3.6
通讯作者：
Tung,HYL
中科院分区：
文献类型：
--
作者：
Zhang,TaoT;Platholi,Jimcy;Heerdt,PaulM;HemmingsJr,HughC;Tung,HYL
蛋白磷酸酶-2A (PP-2A) 与细胞死亡途径的多个调节因子相互作用，因此是连接全脑缺血与细胞死亡的信号传导途径的潜在组成部分。使用一种定量 PP-2A 活性的新方法，我们发现心脏骤停伴复苏和再灌注导致 PP-2A 在猪脑提取物中活化 1.6 倍，在部分纯化 PP-2A 后活化 3.4 倍。这是在临床相关的短暂性全脑缺血模型中首次证明 PP-2A 激活。这些结果表明，抑制 PP-2A 活性可能对全脑缺血具有神经保护作用。
Protein phosphatase-2A (PP-2A) interacts with several regulators of cell death pathways and is therefore a potential component of signaling pathways linking global cerebral ischemia to cell death. Using a novel procedure to quantify PP-2A activity, we find that cardiac arrest with resuscitation and reperfusion leads to activation of PP-2A by 1.6-fold in pig brain extract and by 3.4-fold after partial purification of PP-2A. This is the first demonstration of PP-2A activation in a clinically relevant model of transient global cerebral ischemia. These results suggest that inhibition of PP-2A activity may be neuroprotective in global cerebral ischemia.