Anti-inflammatory effects of isopropyl 3-(3, 4-dihydroxyphenyl)-2-hydroxypropanoate, a novel metabolite from danshen, on activated microglia.
Anti-inflammatory effects of isopropyl 3-(3, 4-dihydroxyphenyl)-2-hydroxypropanoate, a novel metabolite from danshen, on activated microglia.
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DOI：
10.4077/cjp.2012.baa045
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发表时间：
2012-12
影响因子：
1.8
通讯作者：
Shao-Xia Wang
中科院分区：
文献类型：
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作者：
Shao-Xia Wang
下调小胶质细胞活化代表了对抗多种脑疾病如中风和神经退行性疾病的实用策略。在本研究中，我们发现3-（3，4-二羟基苯基）-2-羟基丙酸异丙酯（IDHP），丹参（丹参Bunge）的一种新的生物活性代谢产物，在脂多糖（LPS）诱导的小胶质细胞中发挥抗炎作用。结果显示，IDHP可显著降低LPS诱导的BV-2细胞和大鼠原代小胶质细胞中NO、TNF-α和IL-1 β的生成，且呈剂量依赖性。IDHP还可抑制LPS诱导的诱导型一氧化氮合酶（iNOS）、TNF-α和IL-1β的mRNA表达。IDHP还能显著抑制LPS诱导的BV-2细胞活性氧（ROS）的产生、核因子κB（NF-κB）转位和DNA结合活性。这些结果表明，IDHP可能是在治疗各种小胶质细胞介导的神经炎症性疾病的价值。
Down-regulation of microglial activation represents a practical strategy for combating diverse brain disorders such as stroke and neurodegenerative diseases. In the present study, we showed evidence that isopropyl 3-(3, 4-dihydroxyphenyl)-2-hydroxypropanoate (IDHP), a new bioactive metabolite of Danshen (Salvia miltiorrhiza Bunge), exerted an anti-inflammatory effect in lipopolysaccharide (LPS)- induced microglia. Our data showed that IDHP significantly reduced the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in LPS-induced BV-2 cells and rat primary microglia in a dose-dependent manner. IDHP also suppressed mRNA expression of LPSinduced inducible nitric oxide synthase (iNOS), TNF-α and IL-1β. Moreover, IDHP significantly suppressed the production of reactive oxygen species (ROS), nuclear factor κB (NF-κB) translocation and DNA binding activity induced by LPS treatment in BV-2 cells. These findings indicated that IDHP might be of value in the treatment of various microglia-mediated neuroinflammatory diseases.