Effect of Rheum palmatum L. on NF- kappa B signaling pathway of mice with acute liver failure
Effect of Rheum palmatum L. on NF- kappa B signaling pathway of mice with acute liver failure
复制标题
鸡掌大黄对急性肝衰竭小鼠NF-κB信号通路的影响
DOI：
10.1016/j.apjtm.2015.09.011
复制
发表时间：
2015
影响因子：
3.1
通讯作者：
Mao De-Wen
中科院分区：
文献类型：
--
作者：
Zhang Rong-Zhen;Qiu Hua;Wang Na;Long Fu-Li;Mao De-Wen
目的探讨掌叶大黄对急性肾衰小鼠核因子-半乳糖苷酶B(NF-κB)信号通路的调节作用。治疗组和阳性对照组小鼠灌胃给药。并在模型建筑之前分成两部分。正常组小鼠连续3d腹腔注射等量生理盐水，造模16h后，取小鼠眼球血，处死。检测各组小鼠血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、一氧化氮、白介素1β含量及肝组织半胱氨酸天冬氨酸氨基转移酶3、8活性。结果ALF模型组小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(κ)、一氧化氮(NO)、白三烯天冬氨酸氨基转移酶(IL-1)α含量及肝组织半胱氨酸天冬氨酸氨基转移酶3(Caspase3)、半胱氨酸天冬氨酸氨基转移酶8(Caspase8)活性均升高。此外，诱导型一氧化氮合酶、环氧合酶-2和bax的表达增加，增殖细胞核抗原-2和增殖细胞核抗原的表达减少，核因子-κB p65和I-κBα的磷酸化显著，治疗组大鼠脑组织中iNOS、COX-2和bax的表达明显增强。结论通过核因子-κB信号转导通路，可抑制上述变化。能降低ALF小鼠血清中肝功能酶和炎症因子的含量，调节细胞凋亡相关蛋白的表达，改善ALF小鼠的症状。
ObjectiveTo explore the regulation effect ofRheum palmatum(R. palmatum) L. on NF-κB signaling pathway of ALF mice.MethodsThe intraperitoneal injection ofd-GalN/LPS was employed for the model building. Mice in the treatment group and positive control group were given theR. palmatumL. and bifendate before the model building. Mice in the normal group were given the intraperitoneal injection of equivalent normal saline for continuously 3 d. After 16 h of model building, the blood was collected from eyeballs of mice and then mice were executed. The measurement was performed on the content of ALT, AST, NO and Il-1β in the serum of mice in each group, as well as the activity of Caspase 3 and Caspase 8 in the liver tissue. HE staining was employed to detect the pathological morphology of liver; and the western blot was used to detect the expression of iNOS, COX-2, Bax, Bcl-2, PCNA, NF-κB p65 and IκBα.ResultsThe content of ALT, AST, NO and Il-1β in the serum and the activity of Caspase 3 and Caspase 8 in the liver tissue were increased in the mice of ALF model group. Besides, the expression of iNOS, COX-2 and Bax was increased, the expression of Bcl-2 and PCNA was decreased, the phosphorylation of NF-κB p65 and IκBα was significant and the treatment group ofR. palmatumL. could inhibit such change.ConclusionsThrough NF-κB signaling pathway, theR. palmatumL. could reduce the content of enzyme of liver function and inflammation factor in the serum of ALF mice, regulate the expression of cell apoptosis-related protein and improve the symptoms of ALF mice.