Modeling the efficiency of a magnetic needle for collecting magnetic cells.
Modeling the efficiency of a magnetic needle for collecting magnetic cells.
复制标题
对磁针收集磁性细胞的效率进行建模。
DOI：
10.1088/0031-9155/59/13/3319
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发表时间：
2014
影响因子：
3.5
通讯作者：
Flynn,EdwardR
中科院分区：
文献类型：
--
作者：
Butler,KimberlyS;Adolphi,NatalieL;Bryant,HC;Lovato,DebbieM;Larson,RichardS;Flynn,EdwardR
随着基于磁性纳米颗粒的新技术的开发和新靶细胞的鉴定，迫切需要了解流体中特定细胞的磁性分离的重要特征，这是疾病研究和诊断中越来越重要的工具。为了研究磁性细胞收集，将涂覆有超顺磁性纳米颗粒的细胞大小的球形微粒悬浮在（1）甘油-水溶液和（2）水中，所述甘油-水溶液被选择为接近骨髓的粘度范围，在所述水中总悬浮微球的3、5、10和100%涂覆有磁性纳米颗粒，以模拟从流体中的细胞混合物中收集罕见的磁性纳米颗粒涂覆的细胞。磁性微球上收集的磁针，我们证明，收集效率与时间的关系可以使用一个简单的，化学衍生的功能，与三个物理上重要的参数建模。该函数能够缩放实验获得的收集效率，以提取悬浮介质的有效阻力。该分析的结果表明，有效阻力与流体粘度成线性比例，正如预期的那样。令人惊讶的是，增加悬浮流体中非磁性微球的数量导致磁性微球的收集增加，对应于介质的有效阻力的降低。
As new magnetic nanoparticle-based technologies are developed and new target cells are identified, there is a critical need to understand the features important for magnetic isolation of specific cells in fluids, an increasingly important tool in disease research and diagnosis. To investigate magnetic cell collection, cell-sized spherical microparticles, coated with superparamagnetic nanoparticles, were suspended in (1) glycerine–water solutions, chosen to approximate the range of viscosities of bone marrow, and (2) water in which 3, 5, 10 and 100% of the total suspended microspheres are coated with magnetic nanoparticles, to model collection of rare magnetic nanoparticle-coated cells from a mixture of cells in a fluid. The magnetic microspheres were collected on a magnetic needle, and we demonstrate that the collection efficiency versus time can be modeled using a simple, heuristically-derived function, with three physically-significant parameters. The function enables experimentally-obtained collection efficiencies to be scaled to extract the effective drag of the suspending medium. The results of this analysis demonstrate that the effective drag scales linearly with fluid viscosity, as expected. Surprisingly, increasing the number of non-magnetic microspheres in the suspending fluid results increases the collection of magnetic microspheres, corresponding to a decrease in the effective drag of the medium.