DNA Sequencing Sensors: An Overview.
DNA Sequencing Sensors: An Overview.
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DOI：
10.3390/s17030588
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发表时间：
2017-03-14
期刊：
影响因子：
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通讯作者：
Manzano-Agugliaro F
中科院分区：
文献类型：
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作者：
Garrido-Cardenas JA;Garcia-Maroto F;Alvarez-Bermejo JA;Manzano-Agugliaro F
40年前，诺贝尔化学奖赢家弗雷德里克·桑格（Frederick Sanger）首次公布了完整基因组的测序结果。这对应于噬菌体的小尺寸基因组，但从那时起，已经有许多复杂的生物体的DNA被测序。这是由于生物化学和分子遗传学领域以及纳米技术和计算等其他领域的不断进步。如今，基于遗传物质的测序传感器与桑格使用的传感器没有什么关系。大规模测序传感器或新一代测序（NGS）的出现意味着每次试验中能够测序的遗传物质的数量以及每次运行的时间和成本都有了质的飞跃。我们可以想象，即将到来的技术，已经被称为第四代测序，将继续通过增加每次运行的DNA阅读长度来降低试验的成本。如果传感器和检测系统不变得更小、更精确，所有这一切都是不可能的。本文综述了近40年来发展起来的DNA测序传感器。
The first sequencing of a complete genome was published forty years ago by the double Nobel Prize in Chemistry winner Frederick Sanger. That corresponded to the small sized genome of a bacteriophage, but since then there have been many complex organisms whose DNA have been sequenced. This was possible thanks to continuous advances in the fields of biochemistry and molecular genetics, but also in other areas such as nanotechnology and computing. Nowadays, sequencing sensors based on genetic material have little to do with those used by Sanger. The emergence of mass sequencing sensors, or new generation sequencing (NGS) meant a quantitative leap both in the volume of genetic material that was able to be sequenced in each trial, as well as in the time per run and its cost. One can envisage that incoming technologies, already known as fourth generation sequencing, will continue to cheapen the trials by increasing DNA reading lengths in each run. All of this would be impossible without sensors and detection systems becoming smaller and more precise. This article provides a comprehensive overview on sensors for DNA sequencing developed within the last 40 years.