Mycoplasma orale infection affects K+ and Cl- currents in the HSG salivary gland cell line.
Mycoplasma orale infection affects K+ and Cl- currents in the HSG salivary gland cell line.
复制标题
口腔支原体感染影响HSG唾液腺细胞系中的K电流和Cl电流。
DOI：
10.1007/bf02722962
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Kenny,GE
中科院分区：
文献类型：
--
作者：
Izutsu,KT;Fatherazi,S;Belton,CM;Oda,D;Cartwright,FD;Kenny,GE
在人颌下腺细胞系HSG细胞中纵向研究了K+通道和Cl -通道电流与支原体感染状态的关系。K+通道电流因支原体感染的发生而中断：毒蕈碱激活K+通道和通过离子霉素或低压诱导的K+电流反应估计的K+通道表达均下降。离子霉素和低压诱导的反应在Cl -通道中也有类似的下降，但只有后者的下降具有统计学意义。此外，当暴露于低渗介质时，感染细胞中Cl -电流比K+电流更频繁地被激发（63%的病例对0%的病例），这表明支原体感染对K+通道的影响相对大于Cl -通道。这些变化发生在最初感染的细胞中，当用斯帕沙星清除感染时，这些变化得到改善，当细胞再次感染时，这些变化又复发了。这种变化如果发生在体内，预计会导致唾液分泌减少。
The relations between K+channel and Cl−channel currents and mycoplasma infection status were studied longitudinally in HSG cells, a human submandibular gland cell line. The K+channel currents were disrupted by the occurrence of mycoplasma infection: muscarinic activation of K+channels and K+channel expression as estimated by ionomycin- or hypotonically induced K+current responses were all decreased. Similar decreases in ionomycin- and hypotonically induced responses were observed for Cl−channels, but only the latter decrease was statistically significant. Also, Cl−currents could be elicited more frequently than K+currents (63% of cases versus 0%) in infected cells when tested by exposure to hypotonic media, indicating that mycoplasma infection affects K+channels relatively more than Cl−channels. These changes occurred in the originally infected cells, were ameliorated when the infection was cleared with sparfloxacin, and recurred when the cells were reinfected. Such changes would be expected to result in hyposecretion of salivary fluid if they occurredin vivo.