Regulatory activity of the human CD8+ cell subset: a comparison of CD8+ cells from the intestinal lamina propria and blood.
Regulatory activity of the human CD8+ cell subset: a comparison of CD8+ cells from the intestinal lamina propria and blood.
复制标题
人类 CD8 细胞亚群的调节活性：来自肠固有层和血液的 CD8 细胞的比较。
DOI：
10.1002/eji.1830180105
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发表时间：
1988
影响因子：
5.4
通讯作者：
Elson,CO
中科院分区：
文献类型：
--
作者：
Lee,A;Sugerman,H;Elson,CO
本研究旨在更好地确定人肠道固有层（LP）的免疫调节机制。取肠切除术患者外周血（PB）和LP细胞。在筛选技术中使用杂交瘤抗体分离CD4+和CD8+细胞亚群。在自体新鲜B细胞+ CD4+细胞+商陆有丝分裂原的培养中，分别加入不同数量的LP和PB CD8+细胞。体外培养10天后，收集上清液，用同种型特异性夹心ELSA检测IgM和IgA合成。通过5 × lo4B细胞加5 × 104CD4+细胞组成的指示培养，PB和LP CD8+细胞对IgM和IgA合成的抑制程度相当。然而，当将这些CD8+细胞添加到5 × 104B细胞加105CD4+细胞的指示培养物中时，PB CD8+细胞仍然抑制IgM和IgA的合成，但LP CD8+细胞增强了IgM和IgA的合成。在免疫球蛋白合成不理想的培养中，LP而非PB CD8+细胞也增强了IgM和IgA的合成。尽管有这些结果，当LP CD8+细胞与商陆有丝分裂原一起培养时，这两种细胞类型不能帮助B细胞免疫球蛋白的合成。LP CD8+细胞增强免疫球蛋白的机制似乎涉及CD4+而不是CD8+抑制细胞的拮抗作用。我们得出的结论是，在LP CD8+细胞中，功能异质性更为明显，抑制因子和反抑制因子都有活性，后者在LP细胞中表现出主要活性，而在PB CD8+细胞中则没有。
This study was done to better define the immunoregulatory mechanisms in the human intestinal lamina propria (LP). Peripheral blood (PB) and LP cells were obtained from patients having intestinal resections. CD4+and CD8+cell subsets were isolated using hybridoma antibodies in a panning technique. Graded numbers of LP and PB CD8+cells were added to cultures of autologous fresh B cells plus CD4+cells plus pokeweed mitogen. After 10 days incubationin vitro, the supernatants were collected, and IgM and IgA synthesis was measured by isotype‐specific sandwich ELSA. Both PB and LP CD8+cells suppressed IgM and IgA synthesis by indicator cultures consisting of 5 × lo4B cells plus 5 × 104CD4+cells to a comparable extent. However, when these same CD8+cells were added to indicator cultures of 5 × 104B cells plus 105CD4+cells, PB CD8+cells still suppressed, but LP CD8+cells enhanced IgM and IgA synthesis. LP but not PB CD8+cells also augmented IgM and IgA synthesis in cultures with suboptimal immunoglobulin synthesis. Despite these results, LP CD8+cells were not able to provide help for B cell immunoglobulin synthesis when these two cell types were cultured together with pokeweed mitogen. The mechanism of immunoglobulin augmentation by LP CD8+cells appeared to involve antagonism of a CD4+rather than CD8+suppressor cells. We conclude that functional heterogeneity is more evident within the LP CD8+subset, with both suppressor and contra‐suppressor activities demonstrable, with the latter representing a major activity in LP but not in PB CD8+cells.