Effects of intestinal inflammation on specific subgroups of guinea-pig celiac ganglion neurons
Effects of intestinal inflammation on specific subgroups of guinea-pig celiac ganglion neurons
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DOI：
10.1016/j.neulet.2008.08.045
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发表时间：
2008-10
影响因子：
2.5
通讯作者：
Xiao-xia Dong;M. Thacker;L. Pontell;J. Furness;K. Nurgali
中科院分区：
文献类型：
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作者：
Xiao-xia Dong;M. Thacker;L. Pontell;J. Furness;K. Nurgali
研究了豚鼠回肠短区域炎症对腹腔神经节神经元特性的影响。在取组织前6-7天，通过腔内注射三硝基苯磺酸盐在5- 8 cm的肠中诱导炎症（回肠炎）。腹腔神经节神经元进行了研究，使用细胞内微电极和细胞充满染料从记录电极，以确定其形态。鉴定了紧张性和阶段性神经元。在正常豚鼠和回肠炎豚鼠的神经节中，强直性神经元的胞体较大，树突较长，数量较多。紧张性神经元选择性地受到肠道炎症的影响。回肠炎症后相同强度的去极化电流诱发的动作电位数目是对照组的2倍，诱发动作电位的阈值电流约为对照组的一半，部分神经元自发活动。来自未治疗或假手术动物的神经元从未自发活动。更多的神经元受到影响，而不是投射到发炎的5- 8厘米的肠道。我们的结论是，炎症的一段回肠导致选择性的，体液介导的，增加兴奋性的强直神经元的腹腔神经节，控制运动和分泌，但不是阶段性神经元，项目的肠血管和其他目标。
The consequences of inflammation of a short region of the guinea-pig ileum on the properties of neurons in the celiac ganglia were investigated. Inflammation (ileitis) was induced in 5–8cm of intestine by the intralumenal injection of trinitrobenzene sulfonate, 6–7 days before tissue was taken. Celiac ganglion neurons were investigated using intracellular microelectrodes and the cells were filled with dye from the recording electrode, to determine their morphologies. Tonic and phasic neurons were identified. In ganglia from normal guinea-pigs and from guinea-pigs with ileitis, cell bodies of tonic neurons were larger and their dendrites were longer and more numerous than those of phasic neurons. Tonic neurons were selectively affected by intestinal inflammation. The number of action potentials elicited by the same intensity of depolarizing current for neurons after ileal inflammation was twice that of neurons from control animals, the threshold current to evoke action potentials was about half, and some of the neurons were spontaneously active. Neurons from untreated or sham-operated animals were never spontaneously active. Many more neurons were affected than project to the 5–8cm of intestine that was inflamed. We conclude that inflammation of a segment of the ileum causes a selective, humorally mediated, increase in excitability of tonic neurons in the celiac ganglion that control motility and secretion, but not of phasic neurons that project to the intestinal vasculature and other targets.