Establishment of a rat placental cell line expressing characteristics of extraembryonic membranes.
Establishment of a rat placental cell line expressing characteristics of extraembryonic membranes.
复制标题
表达胚外膜特征的大鼠胎盘细胞系的建立。
DOI：
10.1016/0012-1606(87)90466-0
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
Andrews,GK
中科院分区：
文献类型：
--
作者：
Soares,MJ;Schaberg,KD;Pinal,CS;De,SK;Bhatia,P;Andrews,GK
A cell line was derived from midgestation chorioallantoic placental explants of the outbred Holtzman rat. The cell line was found to express characteristics of extraembryonic membranes and to grow when introduced into allogeneic hosts. Growth in allogeneic hosts was detected following intraperitoneal injection of the cells but not following subcutaneous injection. The transplanted cells grew as cystic structures free in the peritoneum and as solid masses adhered to various abdominal organs. Cystic structures had a homogeneous morphology consisting of an epithelial-like cell layer surrounding a fluid-filled sac. Solid masses had a heterogeneous morphology, containing parts resembling normal components of the extraembryonic membranes (trophoblast, parietal, and visceral yolk sacs). Biochemical analysis of the placenta-derived cell line and transplanted structures derived from the cell line indicated that the cells had the potential to produce a variety of proteins characteristic of extraembryonic tissues. Cultured cells and both types ofin vivotransplants produced the basement membrane protein, laminin. Peritoneal cystic structures also contained α-fetoprotein mRNA and very high levels ofc-fosmRNA. Solid masses demonstrated elevated alkaline phosphatase activity, a marker of trophoblast cells. Cells grownin vitroexpressed elevated c-mycmRNA levels, whereas, c-mycmRNA levels were reduced in thein vivotransplants. The behavior of the cell linein vitroand followingin vivotransplantation suggests it contains elements capable of differentiation toward various components of the extraembryonic membranes. The results indicate that the rat placental cell line will be valuable for future studies on the differentiation of trophoblast cells and other components of the extraembryonic membranes.
