SNAREs and the specificity of membrane fusion
SNAREs and the specificity of membrane fusion
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DOI：
10.1016/s0962-8924(01)01929-8
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发表时间：
2001-03-01
影响因子：
19
通讯作者：
Pelham, HRB
中科院分区：
文献类型：
--
作者：
Pelham, HRB
细胞内膜运输的一个主要问题涉及运输囊泡找到并与其靶细胞器融合的方式。SNARE蛋白参与融合，它们的相互识别原则上可以提供必要的特异性。或者，由外周膜蛋白介导的囊泡的初步束缚可能是关键。以前的研究表明，SNARE复合物在溶液中的组装几乎没有特异性，但最近的实验表明，酵母SNARE锚定在脂质体中的相互作用可以是高度选择性的。栓系和SNARE接合可能都有助于膜转运的准确性。
A major problem of intracellular membrane traffic concerns the way in which transport vesicles find and fuse with their target organelles. SNARE proteins are involved in fusion, and their mutual recognition could in principle provide the necessary specificity. Alternatively, the preliminary tethering of vesicles, mediated by peripheral membrane proteins, could hold the key. Previous studies of SNARE complex assembly in solution have suggested little specificity, but recent experiments with yeast SNAREs anchored in liposomes show that their interactions can be highly selective. It is likely that both tethering and SNARE engagement contribute to the accuracy of membrane transport.