Liquid-crystal based optical sensors for simultaneous detection of multiple glycine oligomers with micromolar concentrations
Liquid-crystal based optical sensors for simultaneous detection of multiple glycine oligomers with micromolar concentrations
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DOI：
10.1016/j.snb.2007.04.040
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发表时间：
2007-11-15
影响因子：
8.4
通讯作者：
Yang, Kun-Lin
中科院分区：
文献类型：
--
作者：
Bi, Xinyan;Huang, Shisheng;Yang, Kun-Lin
本文报道了一种基于液晶的新型方法，用于同时光学检测多种甘氨酸低聚物。检测原理基于向列液晶 4-氰基-4'-戊基联苯 (5CB) 方向的破坏，该向列液晶 4-氰基-4'-戊基联苯 (5CB) 支撑在带有多种甘氨酸低聚物图案的表面上。当不同分子长度的甘氨酸低聚物在优化条件下固定在醛修饰的表面上时。它们形成具有与其分子长度相对应的不同厚度的单层。因此，当厚度超过 0.5 +/- 0.1 nm 时，支撑在表面上的 5CB 的方向会受到干扰。由于液晶是双折射材料，其方向的破坏会导致肉眼可见的光信号。这种基于液晶的甘氨酸低聚物检测方案能够检测 10 μM 的三甘氨酸、四甘氨酸和五甘氨酸、100 μM 的二甘氨酸和 1 mM 的甘氨酸，样品量仅为 2 μL。它还在大面积上提供了良好的空间分辨率，并且非常简单。 (c) 2007 Elsevier B.V. 保留所有权利。
This paper reports a novel liquid-crystal based method for the optical detection of multiple glycine oligomers simultaneously. The detection principle is based on the disruption of the orientations of a nematic liquid crystal 4-cyano-4'-pentylbiphenyl (5CB) supported on a surface patterned with multiple glycine oligomers. When glycine oligomers with different molecular lengths are immobilized on aldehyde-decorated surfaces under optimized conditions. they form monolayers with different thicknesses corresponding to their molecular lengths. As a result, the orientations of 5CB supported on the surfaces are disturbed when the thickness exceeds 0.5 +/- 0.1 nm. Because liquid crystals are birefringent materials, the disruption of their orientations can cause optical signals visible to the naked eye. This liquid-crystal based detection scheme for glycine oligomers is able to C detect 10 mu M of triglycine, tetraglycine and pentaglycine, 100 mu M of diglycine, and 1 mM of glycine with a sample size as little as 2 mu L. It also provides a good spatial resolution over a large area and is sufficiently simple. (c) 2007 Elsevier B.V. All rights reserved.