Evaluations of epithelial sealing and peri-implant epithelial down-growth around "step-type" implants
Evaluations of epithelial sealing and peri-implant epithelial down-growth around "step-type" implants
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DOI：
10.1111/j.1600-0501.2011.02163.x
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发表时间：
2012-04-01
影响因子：
4.3
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Atsuta, Ikiru;Ayukawa, Yasunori;Koyano, Kiyoshi
目的：能够刺激和整合上皮伤口愈合过程的种植体设计可能会显著提高牙种植体的疗效。材料和方法：取大鼠右侧上颌第一磨牙，植入不同高度和/或宽度的直型或阶梯型种植体(N-S：0.8 mm高，0.1 mm宽；W-S：0.8 mm高，0.2 mm宽；W-S：0.8 mm高，0.2 mm宽；H-S：0.4 mm高，0.1 mm宽)。在16周内的不同时间点上颌骨取材，评估层粘连蛋白-5的分布作为伤口愈合和馅饼形成的指示器，马萝卜过氧化物酶(HRP)渗透率作为衡量上皮封闭的指标，以及馅饼的向下生长的形成。结果：在所有种植模型中，馅饼由口腔沟上皮形成，并沿种植体表面顶部扩散。在Ws组，4周时HRP仅见于PIE的冠状区域，而在直型PIE中，HRP仅见于根尖区和结缔组织。16周时，Ws种植体的下垂明显少于Con、NS或HS种植体，与自然牙中观察到的结果相当。结论：阶梯式种植体系统可能具有改善组织-种植体界面上皮封闭的潜力，并减少根尖下垂，从而提高牙种植体的效果。
Objective: Implant designs that can stimulate and integrate with an epithelial wound-healing process may significantly enhance the efficacy of dental implants. Here, we evaluated the potential of step-type implant systems to improve the sealing between the peri-implant epithelium (PIE) and the implant surface, and investigated the effect of implant structure on PIE down-growth.Materials and methods: Right maxillary first molars were extirpated from rats and implanted with either a straight-type or a step-type implant varying in step height and/ or width (N-s: 0.8 mm height, 0.1 mm width; W-s: 0.8 mm height, 0.2 mm width; H-s: 0.4 mm height, 0.1 mm width). Maxillae were harvested at various time points over 16 weeks to evaluate laminin-5 distribution as an indicator of wound healing and PIE formation, horse-radish peroxidase (HRP) penetration as a measurement of epithelial sealing, and PIE down-growth formation.Results: In all implant models, the PIE formed from the oral sulcular epithelium and spread apically along the implant surface. In the Ws group, HRP penetration was detected only in the coronal region of the PIE at 4 weeks, whereas in the straight-type, it was observed in the apical region and the connective tissue. At 16 weeks, the Ws implants exhibited markedly less PIE down-growth than the Con, Ns or Hs implants, and were equivalent to that observed in natural teeth.Conclusion: The step-type implant system may have the potential for improving epithelial sealing at the tissue-implant interface, as well as reducing apical PIE down-growth, thus enhancing dental implant efficacy.