Nutritional status alters saccharin intake and sweet receptor mRNA expression in rat taste buds
Nutritional status alters saccharin intake and sweet receptor mRNA expression in rat taste buds
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营养状况改变大鼠味蕾中糖精摄入量和甜味受体 mRNA 表达
DOI：
10.1016/j.brainres.2010.02.026
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发表时间：
2010-04-14
期刊：
影响因子：
2.9
通讯作者：
Lv, Bo
中科院分区：
文献类型：
--
作者：
Chen, Ke;Yan, Jianqun;Lv, Bo
甜味通常表示有高热量的食物。人们普遍认为，甜味感知、偏好和营养状况之间存在密切的联系。然而，所涉及的机制仍然未知。为了研究营养状况是否会影响大鼠对美味溶液的偏好和改变甜味受体基因表达，我们使用双瓶偏好测试测量了高脂肪饮食诱导的肥胖大鼠和长期饮食限制大鼠的糖精摄入量和偏好，以及体重、血浆瘦素水平和味蕾中甜味受体基因表达的变化。我们发现高脂饮食诱导的肥胖大鼠对0.01 M和0.04 M糖精的消耗和偏好比正常饮食大鼠明显降低，而肥胖大鼠血清瘦素水平明显升高。与糖精摄入量的变化一致，高脂饮食诱导的肥胖大鼠的甜味受体T1R3基因表达水平与对照大鼠相比显著降低。相比之下，长期限制饮食的大鼠对0.04 M糖精的消耗和偏好显著增强。血清瘦素浓度降低，味蕾中瘦素受体基因表达明显升高。总之，我们的研究结果表明，营养状况改变了味蕾中糖精偏好和T1R3的表达。这些过程可能涉及外周甜味敏感性调节的机制，瘦素在其中起作用。(C) 2010 Elsevier B.V.版权所有
Sweet taste usually signifies the presence of caloric food. It is commonly accepted that a close association exists among sweet taste perception, preference, and nutritional status. However, the mechanisms involved remain unknown. To investigate whether nutritional status affects the preference for palatable solutions and alters sweet taste receptor gene expression in rats, we measured saccharin intake and preference using a two-bottle preference test, and changes in body weight, plasma leptin levels, and gene expression for the sweet taste receptor in taste buds in high-fat diet-induced obese rats and chronically diet-restricted rats. We found that the consumption and preference ratios for 0.01 and 0.04 M saccharin were significantly lower in the high-fat diet-induced obese rats than in the normal diet rats, while the serum leptin levels were markedly increased in obese rats. Consistent with the changes in saccharin intake, the gene expression level of the sweet taste receptor T1R3 was significantly decreased in the high-fat diet-induced obese rats compared with the control rats. By contrast, the chronically diet-restricted rats showed remarkably enhanced consumption and preference for 0.04 M saccharin. The serum leptin concentration was decreased, and the gene expression of the leptin receptor was markedly increased in the taste buds. In conclusion, our results suggest that nutritional status alters saccharin preference and the expression of T1R3 in taste buds. These processes may be involved in the mechanisms underlying the modulation of peripheral sweet taste sensitivity, in which leptin plays a role. (C) 2010 Elsevier B.V. All rights reserved.