KLUYVEROMYCES-LACTIS KILLER TOXIN INHIBITS ADENYLATE-CYCLASE OF SENSITIVE YEAST-CELLS
KLUYVEROMYCES-LACTIS KILLER TOXIN INHIBITS ADENYLATE-CYCLASE OF SENSITIVE YEAST-CELLS
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DOI：
10.1038/304464a0
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发表时间：
1983-01-01
期刊：
影响因子：
64.8
通讯作者：
TAMURA, G
中科院分区：
文献类型：
--
作者：
SUGISAKI, Y;GUNGE, N;TAMURA, G
由酿酒酵母K+1菌株分泌的K1杀伤毒素已被充分表征1，2。它是一种分子量（MW）为11，470的简单蛋白质（参考文献3），由称为mRNA的双链线性RNA质粒编码，分子量为1.1-1.7 × 106（参考文献4-6）。对不携带M RNA的敏感酵母菌有致死作用。K+和ATP的泄漏是敏感细胞的第一个明显反应7 -9，毒性作用被认为是由于其作为质子载体10，11或K+离子载体2的作用。最近，在乳酸克鲁维酵母IFO 1267中发现了另一种杀伤毒素，它与双链线性DNA质粒pGK 1 -1（MW 5.4 × 106）和pGK 1 -2（MW 8.4 × 106）1 - 2的存在有关。通过消除pGK 1 -1或缺失作图，已经表明杀伤毒素的结构基因和免疫决定基因位于较小的质粒上13，14。此外，质粒可以从K. lactistoS，原生质体融合和原生质体转化13，15.作为K。乳酸毒素是由DNA质粒编码的，并且具有比K1杀伤毒素相对更宽的作用谱12，13，该毒素的作用模式是非常令人感兴趣的。在这里，我们报告说，K。乳酸菌素抑制敏感酵母细胞中的腺苷酸环化酶，并使细胞停滞在G1期。
K1killer toxin secreted by the K+1strain ofSaccharomyces cerevisiae, has been well characterized1,2. It is a simple protein of molecular weight (MW) 11,470 (ref. 3), encoded by a double-stranded, linear RNA plasmid, called MRNA, of MW 1.1–1.7 × 106(refs 4–6). It is lethal to sensitiveSaccharomyces cerevisiaewhich does not carry M RNA. Leakage of K+and ATP is the first distinct response in sensitive cells7–9, and the toxic action is thought to be due to its action as a protonophore10,11or K+ionophore2. Recently, a further killer toxin has been found inKluyveromyces lactisIFO 1267, and it is associated with the presence of the double-stranded linear DNA plasmids, pGK1-1 (MW 5.4 × 106) and pGK1-2 (MW 8.4 × 106)12. It has been shown, by curing pGK1-1 or deletion mapping, that the structural gene for the killer toxin and immunity-determining gene reside on the smaller plasmid13,14. Moreover, the plasmids could be transferred fromK. lactistoS. cerevisiaeby protoplast fusion and protoplast transformation13,15. As theK. lactistoxin is encoded by a DNA plasmid and has a relatively wider action spectrum than K1killer toxin12,13, the mode of action of the toxin is highly interesting. Here we report thatK. lactistoxin inhibits adenylate cyclase in sensitive yeast cells and brings about arrest of the cells at the G1stage.