THE TESTICULAR RECEPTOR FOR FOLLICLE-STIMULATING-HORMONE - STRUCTURE AND FUNCTIONAL EXPRESSION OF CLONED CDNA
THE TESTICULAR RECEPTOR FOR FOLLICLE-STIMULATING-HORMONE - STRUCTURE AND FUNCTIONAL EXPRESSION OF CLONED CDNA
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DOI：
10.1210/mend-4-4-525
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发表时间：
1990-04-01
影响因子：
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通讯作者：
SEEBURG, PH
中科院分区：
文献类型：
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作者：
SPRENGEL, R;BRAUN, T;SEEBURG, PH
从同源文库中分离出编码大鼠支持细胞 FSH 受体的克隆 cDNA，并在培养的哺乳动物细胞中进行功能表达。根据 cDNA 预测，FSH 受体 (FSH-R) 是一个 75K 的单一多肽，具有 348 个残基的胞外结构域，其中包含三个 N 连接糖基化位点。该结构域与包含七个假定跨膜片段的结构相连，该结构显示出与 G 蛋白偶联受体的序列相似性。因此，FSH-R 的结构设计与 LH/CG 受体 (LH/CG-R) 相同。此外，两种受体在其大的胞外结构域中显示出 50% 的序列相似性，并且在七个跨膜片段中显示出 80% 的同一性。克隆的 cDNA 在哺乳动物细胞中的表达导致 FHS 依赖性 cAMP 积累。 FSH 的选择性由以下事实证明：相关的人糖蛋白激素人 CG 和人 TSH 不会刺激 FSH-R 表达细胞中的腺苷酸环化酶，即使这些激素以高浓度存在。
Cloned cDNA encoding the rat Sertoli cell receptor for FSH was isolated from a cognate library and functionally expressed cultured mammalian cells. The FSH receptor (FSH-R), as predicted from the cDNA, is a single 75K polypeptide with a 348 residue extracellular domain which contains three N-linked glycosylation sites. This domain is connected to a structure containing seven putative transmembrane segments which displays sequence similarity to G protein-coupled receptors. Thus, the FSH-R is identical in its structural design to the LH/CG receptors (LH/CG-R). Furthermore, both receptors display 50% sequence similarity in their large extracellular domains and 80% identity across the seven transmembrane segments. Expression of the cloned cDNA in mammalian cells conferred FHS-dependent cAMP accumulation. The selectivity for FSH is attested by the fact that the related human glycoprotein hormones human CG and human TSH do not stimulate adenylyl cyclase in FSH-R expressing cells even when these hormones are present at high concentrations.