Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions:: Temporal expression of genes for key sigma factors
Growth and sporulation of Bacillus cereus ATCC 14579 under defined conditions:: Temporal expression of genes for key sigma factors
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DOI：
10.1128/aem.70.4.2514-2519.2004
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发表时间：
2004-04-01
影响因子：
4.4
通讯作者：
Abee, T
中科院分区：
文献类型：
--
作者：
de Vries, YP;Hornstra, LM;Abee, T
一个气升式发酵罐系统，允许精确调节pH值和通气与化学成分确定的培养基相结合，用于研究蜡样芽孢杆菌ATCC 14579的生长和孢子形成。孢子形成完全且同步。sigA，sigB，sigF，和sigG的表达进行了监测与实时逆转录-PCR，和模式定性类似的枯草芽孢杆菌。该方法允许稳定孢子的可再现生产，同时同步生长和限定的条件非常适合于B的细胞分化的进一步基因表达研究。蜡状。
An airlift fermentor system allowing precise regulation of pH and aeration combined with a chemically defined medium was used to study growth and sporulation of Bacillus cereus ATCC 14579. Sporulation was complete and synchronous. Expression of sigA, sigB, sigF, and sigG was monitored with real-time reverse transcription-PCR, and the pattern qualitatively resembled that of Bacillus subtilis. This method allows reproducible production of stable spores, while the synchronous growth and defined conditions are excellently suitable for further gene expression studies of cellular differentiation of B. cereus.