Circulating miRNAs in Pediatric Pulmonary Hypertension Show Promise as Biomarkers of Vascular Function.
Circulating miRNAs in Pediatric Pulmonary Hypertension Show Promise as Biomarkers of Vascular Function.
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DOI：
10.1155/2017/4957147
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发表时间：
2017
影响因子：
--
通讯作者：
Shandas R
中科院分区：
文献类型：
--
作者：
Kheyfets VO;Sucharov CC;Truong U;Dunning J;Hunter K;Ivy D;Miyamoto S;Shandas R
本研究的目的是评估循环mirna作为儿童肺动脉高压血管功能生物标志物的效用。14例小儿肺动脉高压患者同时行右心导管（RHC）和血液生化分析。使用单变量和逐步多变量线性回归来确定反应性和电阻性后负荷与循环miRNA水平的相关性。此外，循环miRNA候选物根据对氧气（O2）或吸入一氧化氮（iNO）反应后电阻性负荷降低20%对患者进行分类，并使用受体操作曲线进行鉴定。32种循环mirna与肺血管阻力指数（PVRi）、肺动脉扩张性以及PVRi在O2和/或iNO作用下的降低相关。多变量模型结合了多个有希望的miRNA候选物的预测能力，显示了与抗性（r = 0.97, P2 - tail < 0.0001）和反应性（r = 0.86, P2 - tail < 0.005）后负荷的良好相关性。Bland-Altman图显示，对于电阻性和反应性后负荷，多变量模型与RHC之间95%的差异分别在0.13 (mmHg - min/L)m2和0.0085/mmHg之间。循环miR-663被证明是血管对急性O2和iNO挑战的反应性的良好分类器。这项研究表明，循环mirna可能是儿童PAH血管功能表型的生物标志物。
The objective of this study was to evaluate the utility of circulating miRNAs as biomarkers of vascular function in pediatric pulmonary hypertension. Fourteen pediatric pulmonary arterial hypertension patients underwent simultaneous right heart catheterization (RHC) and blood biochemical analysis. Univariate and stepwise multivariate linear regression was used to identify and correlate measures of reactive and resistive afterload with circulating miRNA levels. Furthermore, circulating miRNA candidates that classified patients according to a 20% decrease in resistive afterload in response to oxygen (O2) or inhaled nitric oxide (iNO) were identified using receiver-operating curves. Thirty-two circulating miRNAs correlated with the pulmonary vascular resistance index (PVRi), pulmonary arterial distensibility, and PVRi decrease in response to O2 and/or iNO. Multivariate models, combining the predictive capability of multiple promising miRNA candidates, revealed a good correlation with resistive (r = 0.97, P2−tailed < 0.0001) and reactive (r = 0.86, P2−tailed < 0.005) afterloads. Bland-Altman plots showed that 95% of the differences between multivariate models and RHC would fall within 0.13 (mmHg−min/L)m2 and 0.0085/mmHg for resistive and reactive afterloads, respectively. Circulating miR-663 proved to be a good classifier for vascular responsiveness to acute O2 and iNO challenges. This study suggests that circulating miRNAs may be biomarkers to phenotype vascular function in pediatric PAH.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
7.4
作者：
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通讯作者：
Panelli MC
影响因子：
24
作者：
Douwes, Johannes M.;Humpl, Tilman;Berger, Rolf M. F.
通讯作者：
Berger, Rolf M. F.
影响因子：
2.6
作者：
Kheyfets, Vitaly;Thirugnanasambandam, Mirunalini;Finol, Ender
通讯作者：
Finol, Ender
影响因子：
8
作者：
Grant, Jennifer S.;White, Kevin;MacLean, Margaret R.;Baker, Andrew H.
通讯作者：
Baker, Andrew H.