SIMULTANEOUS MEASUREMENT OF CYTOCHROME P4501A CATALYTIC ACTIVITY AND TOTAL PROTEIN-CONCENTRATION WITH A FLUORESCENCE PLATE READER
SIMULTANEOUS MEASUREMENT OF CYTOCHROME P4501A CATALYTIC ACTIVITY AND TOTAL PROTEIN-CONCENTRATION WITH A FLUORESCENCE PLATE READER
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DOI：
10.1006/abio.1994.1476
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发表时间：
1994-10-01
影响因子：
2.9
通讯作者：
JONES, SP
中科院分区：
文献类型：
--
作者：
KENNEDY, SW;JONES, SP
描述了一种同时测量肝微粒体中细胞色素P4501 A相关酶、乙氧基试卤灵-O-脱乙基酶（EROD）和总蛋白浓度的方法。在多孔板中进行EROD测定，并在相同的威尔斯孔中用荧光板读数器定量荧光产物（试卤灵）和总蛋白。使用丁胺进行蛋白质测定。(C)1994年出版社出版。
A method for simultaneous measurement of the cytochrome P4501A-associated enzyme, ethoxyresorufin-O-deethylase (EROD), and total protein concentration in liver microsomes is described. EROD assays were carried out in multiwell plates, and the fluorescent product (resorufin) and total proteins were quantified within the same wells with a fluorescence plate reader. Fluorescamine was used for protein assays. (C) 1994 Academic Press, Inc.