Growth-inhibitory effect of heparin on Babesia parasites
Growth-inhibitory effect of heparin on Babesia parasites
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DOI：
10.1128/aac.48.1.236-241.2004
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发表时间：
2004-01-01
影响因子：
4.9
通讯作者：
Igarashi, I
中科院分区：
文献类型：
--
作者：
Bork, S;Yokoyama, N;Igarashi, I
我们检测了三种肝素对巴贝斯虫生长的抑制作用。光镜观察表明，肝素对牛巴贝斯虫、双双巴贝斯虫、马巴贝斯虫和卡巴氏巴贝斯虫的体外增殖和体内微小巴贝斯虫的增殖均有明显的抑制作用。用不同浓度的肝素处理后，细胞内寄生虫完全清除。有趣的是，在低浓度肝素存在的情况下，观察到异常分裂的牛巴氏杆菌寄生虫的比例更高。此外，激光共聚焦扫描显微镜检测到胞外裂殖子表面较好地发现了荧光素标记的异硫氰酸酯标记的肝素。这些发现表明，肝素覆盖了裂殖子的表面，并抑制了它们随后对红细胞的入侵。
We examined the inhibitory effects of three heparins on the growth of Babesia parasites. The multiplication of Babesia bovis, B. bigemina, B. equi, and B. caballi in in vitro cultures and that of B. microti in vivo were significantly inhibited in the presence of heparins, as determined by light microscopy. Treatment with various concentrations of heparin showed complete clearance of the intracellular parasites. Interestingly, a higher percentage of abnormally multidividing B. bovis parasites was observed in the presence of low concentrations of heparin. Furthermore, fluorescein isothiocyanate-labeled heparin was preferably found on the surfaces of extracellular merozoites, as detected by confocal laser scanning microscopy. These findings indicate that the heparin covers the surfaces of babesial merozoites and inhibits their subsequent invasion of erythrocytes.