Acetyicholinesterase Biosensor Based On Mesoporous Hollow Carbon Spheres/Core-Shell Magnetic Nanoparticles-Modified Electrode for the Detection of Organophosphorus Pesticides
Acetyicholinesterase Biosensor Based On Mesoporous Hollow Carbon Spheres/Core-Shell Magnetic Nanoparticles-Modified Electrode for the Detection of Organophosphorus Pesticides
复制标题
基于介孔空心碳球/核壳磁性纳米颗粒修饰电极的乙酰胆碱酯酶生物传感器用于检测有机磷农药
DOI：
10.3390/s18124429
复制
发表时间：
2018-12-01
期刊：
影响因子：
3.9
通讯作者：
Wang, Hongsu
中科院分区：
文献类型：
--
作者：
Luo, Ruiping;Feng, Zijie;Wang, Hongsu
本研究研究了介孔中空碳球（MHCS）和具有核壳结构的磁性介孔中空碳球（Fe3O4@MHCS）的合成。制备了两种基于介孔碳材料的乙酰胆碱酯酶传感器(乙酰胆碱酯酶/介孔中空碳球/玻璃碳电极（AChE/MHCS/GCE）和乙酰胆碱酯酶/核壳磁性介孔中空碳球/玻璃碳电极（AChE/Fe3O4@MHCS/GCE）。在最佳条件下，以马拉硫磷为模型化合物研制的生物传感器检测范围宽、检出限低、重现性好、稳定性高。在马拉硫磷浓度为0.01 ~ 100 ppb和100 ~ 600 ppb的条件下，AChE/MHCS/GCE电化学传感器在10 min的孵育时间内呈现出两个良好的线性范围，检出限为0.0148 ppb （S/N = 3）。AChE/Fe3O4@MHCS/GCE电化学传感器在马拉硫磷浓度范围为0.01 ~ 50 ppb和50 ~ 600 ppb，孵育时间为12 min，检测限为0.0182 ppb （S/N = 3）。此外，AChE/MHCS/GCE和AChE/Fe3O4@MHCS/GCE生物传感器对实际样品的检测效果良好，适用于有机磷农药（OP）残留的现场检测。
The present study investigated the synthesis of mesoporous hollow carbon spheres (MHCS) and magnetic mesoporous hollow carbon spheres with core-shell structures (Fe3O4@MHCS). Two acetylcholinesterase sensors (acetylcholinesterase/mesoporous hollow carbon spheres/glassy carbon electrode (AChE/MHCS/GCE) and acetylcholinesterase/core-shell magnetic mesoporous hollow carbon spheres/glassy carbon electrode (AChE/Fe3O4@MHCS/GCE) based on mesoporous carbon materials were prepared. Under the optimum conditions, using Malathion as the model compound, the developed biosensors showed a wide detection range, low detection limit, good reproducibility, and high stability. The AChE/MHCS/GCE electrochemical sensor response exhibited two good linear ranges at the incubation time of 10 min at the Malathion concentration ranges of 0.01 to 100 ppb and 100 to 600 ppb, with a detection limit of 0.0148 ppb (S/N = 3). The AChE/Fe3O4@MHCS/GCE electrochemical sensor that was operated with an incubation time of 12 min at the malathion concentration ranges between 0.01-50 ppb and 50-600 ppb had a detection limit of 0.0182 ppb (S/N = 3). Moreover, the AChE/MHCS/GCE and AChE/Fe3O4@MHCS/GCE biosensors were effective for the detection of real samples, and were demonstrated to be suitable for the field-testing of organophosphorus pesticide (OP) residues.