Antioxidant and antigenotoxic properties of CeO2 NPs and cerium sulphate: Studies with Drosophila melanogaster as a promising in vivo model
Antioxidant and antigenotoxic properties of CeO2 NPs and cerium sulphate: Studies with Drosophila melanogaster as a promising in vivo model
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DOI：
10.3109/17435390.2014.976284
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发表时间：
2015-01-01
期刊：
影响因子：
5
通讯作者：
Marcos, Ricard
中科院分区：
文献类型：
--
作者：
Alaraby, Mohamed;Hernandez, Alba;Marcos, Ricard
虽然体外方法最常用于测试纳米材料的潜在有害影响，但体内研究产生了相关信息，补充了体外数据。在这种情况下，我们提倡使用果蝇作为合适的体内模型，以评估与纳米材料暴露相关的潜在风险。本研究的主要目的是评估氧化铈纳米颗粒（Ce-NPs）和硫酸铈（IV）暴露相关的不同生物学效应。评价的终点是卵至成虫的存活率、通过肠屏障的颗粒摄取、血细胞的基因表达和细胞内活性氧（ROS）产生、遗传毒性和抗遗传毒性。透射电子显微镜图像显示肠屏障和血细胞的Ce-NPs的内化，并检测到热休克蛋白基因的显着表达。尽管有这些发现，但未观察到与两种形式铈相关的毒性或遗传毒性。有趣的是，Ce-NPs显着降低重铬酸钾的遗传毒性作用和细胞内ROS的产生。幼虫处理后未检测到形态畸形。本研究强调了D.在研究由纳米颗粒材料引起的不同生物学效应时，将黑腹动物作为动物模型，这表明其在研究肠屏障在通过摄入进入的纳米材料的转位中的作用方面的有用性。
Although in vitro approaches are the most used for testing the potential harmful effects of nanomaterials, in vivo studies produce relevant information complementing in vitro data. In this context, we promote the use of Drosophila melanogaster as a suitable in vivo model to characterise the potential risks associated to nanomaterials exposure. The main aim of this study was to evaluate different biological effects associated to cerium oxide nanoparticles (Ce-NPs) and cerium (IV) sulphate exposure. The end-points evaluated were egg-to-adult viability, particles uptake through the intestinal barrier, gene expression and intracellular reactive oxygen species (ROS) production by haemocytes, genotoxicity and antigenotoxicity. Transmission electron microscopy images showed internalisation of Ce-NPs by the intestinal barrier and haemocytes, and significant expression of Hsp genes was detected. In spite of these findings, neither toxicity nor genotoxicity related to both forms of cerium were observed. Interestingly, Ce-NPs significantly reduced the genotoxic effect of potassium dichromate and the intracellular ROS production. No morphological malformations were detected after larvae treatment. This study highlights the importance of D. melanogaster as animal model in the study of the different biological effects caused by nanoparticulated materials, at the time that shows its usefulness to study the role of the intestinal barrier in the transposition of nanomaterials entering via ingestion.