Expression of transforming growth factor-β1 in penile tissue from rats with bilateral cavernosal nerve ablation
Expression of transforming growth factor-β1 in penile tissue from rats with bilateral cavernosal nerve ablation
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DOI：
10.1111/j.1464-410x.2004.04969.x
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发表时间：
2004-08-01
影响因子：
4.5
通讯作者：
Tian, BC
中科院分区：
文献类型：
--
作者：
Hu, WL;Hu, LQ;Tian, BC
探讨双侧海绵体神经（CN）消融后大鼠阴茎组织中转化生长因子- β (1) （tgf - β 1）的表达，模拟前列腺切除术中不保留神经的患者。10只成年雄性大鼠（神经切除组）在手术显微镜下无菌切除双侧CN， 6只假手术大鼠作为对照。手术后15周，所有大鼠进行阿波吗啡试验以评估阴茎勃起。收集并制备阴茎标本，用逆转录聚合酶链反应（RT-PCR）、western blot和免疫组化检测tgf - β 1的表达，并用共聚焦显微镜定量分析海绵体中平滑肌与胶原纤维的比例。假手术组大鼠皮下注射阿波啡（100杯/千克）后勃起反应明显，神经切除术后无大鼠出现勃起反应。免疫组织化学、RT-PCR和western blot分析显示，神经切除术后阴茎组织中tgf - β 1的表达明显高于假手术后。多聚甲醛固定后的平滑肌细胞（红色荧光）和胶原纤维（绿色自身荧光）在共聚焦显微镜下清晰地识别出来。神经切除术后海肌体平滑肌与胶原纤维的平均（sem）比值为0.265(0.125)，显著低于假手术组0.760 (0.196)（P < 0.01）。tgf - β 1在阴茎组织中的表达增加，促进胶原蛋白的合成，可能是双侧CN消融引起勃起功能障碍的重要因素之一。类似的病理生理过程可能发生在根治性前列腺切除术后的患者海绵体中。
To explore the expression of transforming growth factor-beta(1) (TGF-beta1) in penile tissue from rats after bilateral cavernosal nerve (CN) ablation, mimicking patients who have had no nerve-sparing during prostatectomy.Ten adult male rats (neurectomy group) had a bilateral CN resection aseptically under an operating microscope, with six sham-operated rats as controls. Fifteen weeks after surgery an apomorphine test was used in all rats to assess penile erection. The penile specimens were then collected and prepared for detecting the expression of TGF-beta1 by reverse transcription-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry, and for quantitative analysis of the ratio of smooth muscle to collagen fibres in the corpus cavernosum with confocal microscopy.All rats in the sham-operated group but none after neurectomy had an erectile response after subcutaneous injection with apomorphine (100 mug/kg). Immunohistochemistry, RT-PCR and western blot analyses showed a significantly higher expression of TGF-beta1 in the penile tissues after neurectomy than after sham surgery. Smooth muscle cells (fluorescing red) and collagen fibres (green autofluorescence) after paraformaldehyde fixation, were clearly identified by confocal microscopy. The fluorescence intensity expressed as the mean (sem) ratio of smooth muscle to collagen fibres in the corpus cavernosum after neurectomy was 0.265 (0.125), significantly lower than that in the sham-operated group, at 0.760 (0.196) (P < 0.01).An increased expression of TGF-beta1 in penile tissue which promotes the synthesis of collagen may be one of the important factors for the erectile dysfunction caused by bilateral CN ablation. Similar pathophysiological processes may occur in the corpus cavernosum of patients after radical prostatectomy.