Wheat Cellular Membrane Thermotolerance Under Heat Stress
Wheat Cellular Membrane Thermotolerance Under Heat Stress
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DOI：
10.1111/j.1439-037x.2009.00398.x
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发表时间：
2010-04-01
影响因子：
3.5
通讯作者：
Lidon, F. C.
中科院分区：
文献类型：
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作者：
Dias, A. S.;Barreiro, M. G.;Lidon, F. C.
小麦的4个基因型。和小麦（Triticum turgidum subsp.）根据其遗传背景多样性选择硬粒小麦开花后进行热胁迫。对膜渗透性、脂质过氧化和脂肪酸（C14：0、C16：0、C16：1c、C16：1t、C18：0、C18：1、C18：2和C18：3）进行定量。非循环光合电子传递的量子产率的估计以及测试系统，以进一步评估类囊体功能的影响。结果表明，面包小麦和硬粒小麦在灌浆期对高温的科普能力存在差异。基因型Sever对膜脂过氧化和膜透性表现出较高的热敏感性，这通过乙烯和MDA产生的增加以及对反式脂肪酸（灌浆中期）的影响来评估。在硬粒小麦基因型中，也发现了差异，TE 9306显示出高的膜稳定性，而膜透性，MDA和乙烯含量没有增加。以这种方式，在该基因型中观察到的TFA变化可能构成了允许脂质定性变化的机制，反映在膜FA的不饱和度水平较低，这是高温下的积极性状。
Four genotypes of Triticum aestivum L. and Triticum turgidum subsp. durum chosen according to their genetic background diversity were subjected to heat stress after anthesis. Membrane permeability, lipid peroxidation and fatty acids (C14:0, C16:0, C16:1c, C16:1t, C18:0, C18:1, C18:2 and C18:3) were quantified. The estimation of the quantum yield of non-cyclic photosynthetic electron transport was used as well as a test system to further evaluate the implications on thylakoid functioning. It was found differences within bread and durum wheat species concerning the capability to cope with high temperatures at the stage of grain filling. The genotype Sever showed high thermal sensitivity concerning membrane lipid peroxidation and membrane permeability, as evaluated by the increased production of ethylene and MDA, as well as by the impact on TFA (at the middle term of grain filling). In the durum wheat genotypes, differences were also found, with TE 9306 displaying high membrane stability, with no increases on membrane permeability, MDA and ethylene content. In this way, the observed changes on TFA in this genotype might have constituted a mechanism to allow qualitative lipid changes, reflected in lower unsaturation level of membrane FAs which is a positive trait under high temperatures.