Nisin resistance in Clostridium botulinum spores and vegetative cells
Nisin resistance in Clostridium botulinum spores and vegetative cells
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DOI：
10.1128/aem.63.7.2654-2659.1997
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发表时间：
1997-07-01
影响因子：
4.4
通讯作者：
Montville, TJ
中科院分区：
文献类型：
--
作者：
Mazzotta, AS;Crandall, AD;Montville, TJ
测定了肉毒梭菌56A、62A、17409A、25763A、213B、B-APHIS和169B的营养细胞和孢子在30℃、每米含乳链菌素0、10(2)、10(3)和10(4)IU的琼脂上形成菌落的频率。菌株56A对链球菌素的抗性频率最高，而菌株62A、169B和B-APHIS的抗性最低，大多数菌株对孢子的抗性高于营养细胞。在一些菌株中，一次Nisin暴露就足以产生稳定的Nisin抗性菌株，逐步增加Nisin浓度可以产生稳定的Nisin抗性菌株，从Nisin抗性菌株产生的孢子保持其Nisin抗性，通过降低培养基的pH和添加3%的NaC l显著降低自发Nisin抗性的频率。链球菌素抗性菌株56A和169B对小儿茶素PA1、巴伐利星MN、植物抗菌素BN和亮氨酸S的抗性也增加。
The frequencies at which vegetative cells and spores of Clostridium botulinum strains 56A, 62A, 17409A 25763A, 213B, B-aphis, and 169B formed colonies on agar media containing 0, 10(2), 10(3), and 10(4) IU of nisin per mi at 30 degrees C were determined. Strain 56A had the highest frequencies of nisin resistance, while strains 62A, 169B, and B-aphis had the lowest, For most strains, spores were more resistant than vegetative cells. One exposure to nisin was sufficient to generate stable nisin-resistant isolates in some strains, Stepwise exposure to increasing concentrations of nisin generated stable resistant isolates from all strains, Spores produced from nisin-resistant isolates maintained their nisin resistance, The frequency of spontaneous nisin resistance was reduced considerably by lowering the pH of the media and adding 3% NaCl. Nisin-resistant isolates of strains 56A and 169B also had increased resistance to pediocin PA1, bavaricin MN, plantaricin BN, and leuconocin S.