Fractionation of high-amylose maize starches by differential alcohol precipitation and chromatography of the fractions
Fractionation of high-amylose maize starches by differential alcohol precipitation and chromatography of the fractions
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DOI：
10.1094/cchem.1998.75.6.887
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发表时间：
1998-11-01
期刊：
影响因子：
2.4
通讯作者：
Thompson, DB
中科院分区：
文献类型：
--
作者：
Klucinec, JD;Thompson, DB
使用异戊醇和l-丁醇对三种高直链玉米淀粉（HAS）和一种普通玉米淀粉（CCS）进行差速醇沉淀，以获得指定为直链淀粉（AM）、支链淀粉（AP）和中间材料（IM）的级分。对于每种淀粉，IM 具有蓝色值和各自 AM 和 AP 的 lambda(max) 之间的碘结合波长最大值 (lambda(max))。尺寸排阻色谱 (SEC) 显示 CCS 和 HAS 的 AM 具有相似性。 HAS AP 比 CCS AP 具有更高的蓝色值和碘结合 lambda(max) 值。与 CCS AP 和 IM 相比，完整 HAS AP 和 IM 的 SEC 均显示在空隙体积后洗脱的材料比例较大 (45-85%)。脱支的 AP 和 IM 的链长 (CL) 分布表明，来自每种淀粉的这些级分是高度支化的，并且 AP 的平均链长比 IM 短。因此，HAS AP 和 IM 的差异沉淀行为似乎取决于一般的支化结构而不是尺寸。我们得出的结论是，在 CCS 和 HAS 中，AP 和 IM 都是支链分子的子集，其中 AP 为主要部分。对于 HAS，AP 和 IM 包含 AM 典型大小的分子，并且包含比 CCS AP 更长的链的比例更高。差速醇沉淀是从 HAS 中分离直链淀粉、支链淀粉和中间材料的有用方法。
Three high-amylose maize starches (HAS) and a common corn starch (CCS) were subjected to differential alcohol precipitation using isoamyl alcohol and l-butanol to obtain fractions designated as amylose (AM), amylopectin (AP), and intermediate material (IM). For each starch, IM had a blue value and an iodine binding wavelength maximum (lambda(max)) between the lambda(max) of the respective AM and AP. Size-exclusion chromatography (SEC) showed similarities in the AM from CCS and HAS. HAS AP had higher blue values and iodine binding lambda(max) values than CCS AP. SEC of the intact HAS AP and IM both showed large proportions of material eluting after the void volume (45-85%) when compared to CCS AP and IM. Chain length (CL) distributions of debranched AP and IM indicated that these fractions from each starch were highly branched, and that AP had a shorter average chain length than IM. Consequently, the differential precipitation behavior of the HAS AP and IM appears dependent on general branching structure rather than size. We conclude that in both CCS and HAS, AP and IM are subsets of the branched molecules with AP as the predominant fraction. For HAS, AP and IM include molecules of a size typical for AM and contain a higher proportion of chains that are longer than those of CCS AP. Differential alcohol precipitation is a useful method of separating amylose, amylopectin, and intermediate material from HAS.