RETINOIC ACID RESPONSIVE GENE-PRODUCT, MIDKINE, HAS NEUROTROPHIC FUNCTIONS FOR MOUSE SPINAL-CORD AND DORSAL-ROOT GANGLION NEURONS IN CULTURE
RETINOIC ACID RESPONSIVE GENE-PRODUCT, MIDKINE, HAS NEUROTROPHIC FUNCTIONS FOR MOUSE SPINAL-CORD AND DORSAL-ROOT GANGLION NEURONS IN CULTURE
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DOI：
10.1002/jnr.490350509
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发表时间：
1993-08-01
影响因子：
4.2
通讯作者：
KIM, SU
中科院分区：
文献类型：
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作者：
MICHIKAWA, M;KIKUCHI, S;KIM, SU
中期因子(MK)是维甲酸应答基因的产物，是一个新的肝素结合生长因子家族的成员。用培养的胎鼠脊髓和背根神经节(DRG)神经元观察MK的神经营养作用。在培养液中加入浓度为1-100 ng/ml的MK，培养7天后，两种神经元的存活率均可提高约5倍。对于脊髓神经元，存活率的增加反映在胆碱乙酰转移酶活性的增加上。MK还可促进脊髓(2倍)和背根节(1.7倍)神经元突起的伸展。MK对这些神经元的促存活活性与碱性成纤维细胞生长因子(BFGF)和白血病抑制因子(LIF)相当。尽管MK对胎鼠神经元有显著影响，但它不能维持出生后小鼠背根神经节神经元的存活。这些结果表明，MK是胚胎脊髓和DRG神经元的神经营养因子，在神经系统的胚胎发生中起重要作用。(C)1993年Wiley-Liss，Inc.
Midkine (MK) is the product of a retinoic acid responsive gene and is a member of a new family of heparin-binding growth factors. Neurotrophic effects of MK were examined using cultured spinal cord and dorsal root ganglion (DRG) neurons derived from fetal mouse. MK, which was added to the culture medium at concentrations of 1-100 ng/ml, promoted survival of both types of neurons approximately 5-fold after 7 days in culture. For spinal cord neurons, the increased survival was reflected in an increase of choline acetyltransferase activity. MK also promoted neurite extension in spinal cord (2-fold) and DRG (1.7-fold) neurons. The survival-promoting activity of MK to these neurons was comparable to that of basic fibroblast growth factor (bFGF) and leukemia inhibitory factor (LIF). In spite of its significant effects on fetal neurons, MK was ineffective in sustaining survival of DRG neurons derived from postnatal mice. From these results, we conclude that MK is a neurotrophic factor to embryonic spinal cord and DRG neurons, and we propose that MK plays a significant role in embryogenesis of the nervous system. (C) 1993 Wiley-Liss, Inc.