QTL mapping of bio-energy related traits in Sorghum
QTL mapping of bio-energy related traits in Sorghum
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DOI：
10.1007/s10681-011-0528-5
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发表时间：
2011-12-01
期刊：
影响因子：
1.9
通讯作者：
Wang, Hong-gang
中科院分区：
文献类型：
--
作者：
Guan, Yan-an;Wang, Hai-lian;Wang, Hong-gang
株高（PHT）、茎叶鲜重（SLFW）、汁重（JW）和茎含糖量（Brix）是甜高粱生物燃料生产的重要性状。使用源自粒用高粱 (Shihong137) x 甜高粱 (L-Tian) 杂交的 F-2 和 F-2:3 群体，通过复合区间作图对 PHT、SLFW、JW 和 Brix 进行 QTL 分析。在四种不同环境下，三个控制PHT的QTL被定位在SBI-01、SBI-07和SBI-09上。这些QTL可以解释10.16%到45.29%的表型变异。在四种环境下一致检测到 SBI-07 和 SBI-09 上的两个主要效应 QTL。 8 个控制 SLFW 的 QTL 在三种环境中进行定位，占表型方差的 5.49-25.36%。在三种环境下观察到SBI-09上位于标记Sb5-206和SbAGE03之间的一个主要QTL。在两种环境下鉴定出4个控制Brix的QTL，占表型变异的11.03-17.65%。在两种环境下检测到6个控制JW的QTL，解释了6.63-23.56%的表型变异。 SBI-07 和 SBI-09 上 JW 的 QTL 在两种环境中是一致的，表现出更高的环境稳定性。此外，我们的研究中还发现了 SBI-07 和 SBI-09 上的两个染色体区域对 PHT、SFLW 和 JW 有重大影响。这些结果将有助于用于生物燃料生产的甜高粱的遗传改良。
Plant height (PHT), stem and leaf fresh weight (SLFW), juice weight (JW) and sugar content of stem (Brix) are important traits for biofuel production in sweet Sorghum. QTL analysis of PHT, SLFW, JW and Brix was conducted with composite interval mapping using F-2 and F-2:3 populations derived from the cross between grain Sorghum (Shihong137) x sweet Sorghum (L-Tian). Three QTLs controlling PHT were mapped on SBI-01, SBI-07 and SBI-09 under four different environments. These QTLs could explain 10.16 to 45.29% of the phenotypic variance. Two major effect QTLs on SBI-07 and SBI-09 were consistently detected under four environments. Eight QTLs controlling SLFW were mapped across three environments and accounted for 5.49-25.36% of the phenotypic variance. One major QTL on SBI-09 located between marker Sb5-206 and SbAGE03 was observed under three environments. Four QTLs controlling Brix were identified under two environments and accounted for 11.03-17.65% of the phenotypic variance. Six QTLs controlling JW were detected under two environments, and explained 6.63-23.56% of the phenotypic variance. QTLs for JW on SBI-07 and SBI-09 were consistent in two environments showing higher environmental stability. In addition, two chromosome regions on SBI-07 and SBI-09 were identified in our study having major effect on PHT, SFLW and JW. The results would be useful for the genetic improvement of sweet Sorghum to be used for biofuel production.