Activation of hepatic stem cell compartment in the rat: role of transforming growth factor alpha, hepatocyte growth factor, and acidic fibroblast growth factor in early proliferation.
Activation of hepatic stem cell compartment in the rat: role of transforming growth factor alpha, hepatocyte growth factor, and acidic fibroblast growth factor in early proliferation.
复制标题
DOI：
--
复制
发表时间：
1993-07
期刊：
影响因子：
--
通讯作者：
R. Evarts;Zongyi Hu;K. Fujio;E. Marsden;Snorri S. Thorgeirsson
中科院分区：
文献类型：
--
作者：
R. Evarts;Zongyi Hu;K. Fujio;E. Marsden;Snorri S. Thorgeirsson
我们之前已经证明肝细胞生长因子（HGF）的表达显著增加（Z. Hu， R. P. Evarts， K. Fujio， E. R. Marsden和S. S. Thorgeirsson, Am）。j .分册。中华肿瘤杂志，2003,19(1)，转化生长因子α (tgf - α) （R. P. Evarts， H. Nakatsukasa， E. R. Marsden, Hu . Z.， S. S. Thorgeirsson, Mol. oncology）。酸性成纤维细胞生长因子（aFGF） （E. R. Marsden， Z. Hu， K. Fujio， H. Nakatsukasa， S. S. Thorgeirsson， R. P. Evarts, Lab.）。投资。肝干细胞和肝周星状细胞（Ito）的增殖和分化与肝干细胞和肝周星状细胞的增殖和分化相一致。在这里，我们使用实验模型研究了大鼠肝脏中干细胞激活的早期阶段，包括乙酰氨基荧光素治疗和部分肝切除术（R. P. Evarts， P. Nagy， E. Marsden和S. S. Thorgeirsson, Carcinogenesis (lod .), 8: 1737-1740, 1987）。干细胞子代和Ito细胞分别用OV6和desmin抗体完成组织化学鉴定。2.1千碱基甲胎蛋白转录本的表达和伴随的DNA合成（[3H]胸苷标签）被用作干细胞区室激活的指标。分析肝部分切除术时及术后4、12、24、48、72、92 h HGF、tgf - α和aFGF的表达。[3H]-胸腺嘧啶标记的OV6和desmin阳性细胞在肝部分切除术后4小时出现在门脉间隙和Glisson胶囊中。（摘要删节250字）
We have demonstrated previously a pronounced increase in the expression of hepatocyte growth factor (HGF) (Z. Hu, R. P. Evarts, K. Fujio, E. R. Marsden, and S. S. Thorgeirsson, Am. J. Pathol., 142: 1823-1830, 1993), transforming growth factor alpha (TGF-alpha) (R. P. Evarts, H. Nakatsukasa, E. R. Marsden, Z. Hu, and S. S. Thorgeirsson, Mol. Carcinog., 5: 25-31, 1992), and acidic fibroblast growth factor (aFGF) (E. R. Marsden, Z. Hu, K. Fujio, H. Nakatsukasa, S. S. Thorgeirsson, and R. P. Evarts, Lab. Invest., 67: 427-433, 1992) that coincided with the proliferation and differentiation of putative hepatic stem cells and perisinusoidal stellate (Ito) cells. Here, we examine the earliest stages of stem cell activation in rat liver using an experimental model involving treatment with acetylaminofluorene and partial hepatectomy (R. P. Evarts, P. Nagy, E. Marsden, and S. S. Thorgeirsson, Carcinogenesis (Lond.), 8: 1737-1740, 1987). Histochemical identification of stem cell progeny and Ito cells was accomplished by OV6 and desmin antibodies, respectively. Expression of the 2.1-kilobase alpha-fetoprotein transcripts and the concomitant DNA synthesis ([3H]thymidine label) were used as indicators for the activation of the stem cell compartment. Expression of HGF, TGF-alpha, and aFGF was analyzed at the time of partial hepatectomy and 4, 12, 24, 48, 72, and 92 h after the operation. [3H]-Thymidine-labeled OV6- and desmin-positive cells were present in the portal space and in the Glisson capsule 4 h after partial hepatectomy.(ABSTRACT TRUNCATED AT 250 WORDS)