Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles.
Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles.
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DOI：
10.3389/fphys.2012.00162
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发表时间：
2012
影响因子：
4
通讯作者：
Kuo WP
中科院分区：
文献类型：
--
作者：
Momen-Heravi F;Balaj L;Alian S;Trachtenberg AJ;Hochberg FH;Skog J;Kuo WP
微泡是体内和体外所有细胞释放的纳米大小的脂质囊泡。它们在正常条件下生理释放，但在肿瘤等病理条件下释放速率更高。一旦释放，它们最终进入体循环，并在血浆、血清、脑脊液、母乳、腹水和尿液等所有生物体液中被发现和表征。微泡代表了它们释放的供体细胞的状态，目前正在对它们作为疾病生物标志物的潜在来源进行深入研究。目前，分离微泡的“黄金标准”是超速离心，尽管已经探索了亲和纯化等替代技术。粘度是流体对剪切应力或拉伸应力产生的变形力的抵抗力。生物流体的不同化学和分子组成会影响其粘度，这可能会影响流体内颗粒的运动。在这篇手稿中，我们解决了粘度是否对超速离心微泡的沉降效率有影响的问题。我们使用不同的生物流体，并在其中掺入聚苯乙烯珠，并使用纳米颗粒跟踪分析评估其回收率。我们证明，MV 回收率与粘度成反比，因此，在处理任何生物流体时，应在超速离心之前考虑样品稀释。
Microvesicles are nano-sized lipid vesicles released by all cells in vivo and in vitro. They are released physiologically under normal conditions but their rate of release is higher under pathological conditions such as tumors. Once released they end up in the systemic circulation and have been found and characterized in all biofluids such as plasma, serum, cerebrospinal fluid, breast milk, ascites, and urine. Microvesicles represent the status of the donor cell they are released from and they are currently under intense investigation as a potential source for disease biomarkers. Currently, the “gold standard” for isolating microvesicles is ultracentrifugation, although alternative techniques such as affinity purification have been explored. Viscosity is the resistance of a fluid to a deforming force by either shear or tensile stress. The different chemical and molecular compositions of biofluids have an effect on its viscosity and this could affect movements of the particles inside the fluid. In this manuscript we addressed the issue of whether viscosity has an effect on sedimentation efficiency of microvesicles using ultracentrifugation. We used different biofluids and spiked them with polystyrene beads and assessed their recovery using the Nanoparticle Tracking Analysis. We demonstrate that MVs recovery inversely correlates with viscosity and as a result, sample dilutions should be considered prior to ultracentrifugation when processing any biofluids.
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影响因子：
1.5
作者：
Raymond, A. D.;Campbell-Sims, T. C.;Powell, M. D.
通讯作者：
Powell, M. D.
DOI：
10.1007/978-1-61779-068-3_15
发表时间：
2011-01-01
期刊：
SERUM/PLASMA PROTEOMICS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Taylor, Douglas D.;Zacharias, Wolfgang;Gercel-Taylor, Cicek
通讯作者：
Gercel-Taylor, Cicek
DOI：
10.1001/jama.1965.03080190030008
发表时间：
1965-01-01
影响因子：
--
作者：
FAHEY, JL;BARTH, WF;SOLOMON, A
通讯作者：
SOLOMON, A
影响因子：
11.2
作者：
Grange, Cristina;Tapparo, Marta;Camussi, Giovanni
通讯作者：
Camussi, Giovanni
影响因子：
11.2
作者：
Di Vizio D;Kim J;Hager MH;Morello M;Yang W;Lafargue CJ;True LD;Rubin MA;Adam RM;Beroukhim R;Demichelis F;Freeman MR
通讯作者：
Freeman MR