RNA synthesis using a universal, base-stable allyl linker.
RNA synthesis using a universal, base-stable allyl linker.
复制标题
使用通用、碱基稳定的烯丙基接头合成 RNA。
DOI：
10.1093/nar/25.20.3980
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发表时间：
1997
影响因子：
14.9
通讯作者：
Jones,RA
中科院分区：
文献类型：
--
作者：
Zhang,X;Gaffney,BL;Jones,RA
The application of a universal allyl linker, 9-O-(4,4′-dimethoxytrityl)-10-undecenoic acid, to the solid phase synthesis of RNA molecules is described. Use of this linker simplifies significantly the isolation and purification steps in RNA synthesis. The linker is universal in that it does not contain a nucleoside. The 3′ terminal nucleoside is instead attached to the support in the first coupling step. The resultant RNA fragment is then obtained as the 3′-phosphate. The linker is basestable, and thus all reagents used during deprotection can simply be washed away, leaving the RNA attached. Further, tritylated short fragments resulting from chain cleavage for any reason are also washed away before separation from the support. This linker is compatible with any current synthetic methodology and any amino functionalized support. Of course, silica supports would not be compatible with fluoride reagents. It could also be used to advantage for other applications. Because it is cleaved under conditions orthogonal to those used during many common reactions, the range of postsynthetic manipulations that can be carried out without cleavage from the support is extended significantly.
