Roles for Potassium and Calcium Channels in the Initiation of Sperm Motility in Rainbow Trout.
Roles for Potassium and Calcium Channels in the Initiation of Sperm Motility in Rainbow Trout.
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钾和钙通道在虹鳟精子活力启动中的作用。
DOI：
10.1111/j.1440-169x.1988.00117.x
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发表时间：
1988
期刊：
影响因子：
4.6
通讯作者：
M. Morisawa
中科院分区：
文献类型：
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作者：
S. Tanimoto;M. Morisawa
众所周知，虹鳟鱼精子的活力受到K+的抑制。我们在这里表明，虽然鳟鱼精子是完全不动的培养基中含有5 mM的K+，运动开始由随后加入几个mM的Ca 2+，这表明K+和Ca 2+的启动精子运动的过程有关。进一步发现，K+通道阻断剂四乙铵、壬基三乙铵、Ba 2+和Cs+以及Ca 2+通道阻断剂维拉帕米在这些试剂抑制其他细胞中通道相关功能的剂量下抑制精子运动的启动。然而，Na+通道阻断剂河豚毒素和阴离子通道阻断剂4，4-二异硫氰基二苯乙烯-2，2 ′-二磺酸仅在极高剂量下抑制运动。这些结果表明，K+和Ca 2+通过离子通道在精子的质膜的运输是第一个事件，触发精子运动的开始在虹鳟鱼。
It is well known that the motility of spermatozoa in rainbow trout is suppressed by K+. We showed here that although trout sperm are completely immotile in medium containing 5 mM K+, motility was initiated by the subsequent addition of several mM Ca2+, suggesting that both K+and Ca2+are related to the process of the initiation of sperm motility. It was further found that K+channel blockers tetraethylammonium, nonyltriethylammonium, Ba2+and Cs+, as well as the Ca2+channel blocker verapamil, inhibited the initiation of sperm motility at doses at which these reagents inhibit chnnel‐related functions in other cells. However, Na+channel blocker, tetrodotoxin and anion channel blocker 4, 4‐diisothiocyatatostilbene‐2, 2′‐disulfonic acid inhibited the motility only at extremely high doses. These results suggest that transport of K+and Ca2+through ion channels at the plasma membrane of spermatozoa is the first event that triggers the initiation of sperm motility in rainbow trout.
从海胆精子中分离出的鞭毛中钠和质子的运输。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lee,HC
通讯作者：
Lee,HC
从海胆精子中分离出的鞭毛中膜电位敏感的 Na -H 交换系统。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lee,HC
通讯作者：
Lee,HC