Identification of Shaker K+ channel family members in Rosaceae and a functional exploration of PbrKAT1
Identification of Shaker K+ channel family members in Rosaceae and a functional exploration of PbrKAT1
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蔷薇科 Shaker K 通道家族成员的鉴定及 PbrKAT1 的功能探索
DOI：
10.1007/s00425-019-03275-3
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发表时间：
2019-12-01
期刊：
影响因子：
4.3
通讯作者：
Wu, Juyou
中科院分区：
文献类型：
--
作者：
Chen, Guodong;Chen, Qian;Wu, Juyou
主要结论爪蟾卵母细胞中的PbrKAT 1蛋白主要在保卫细胞中表达，是一个典型的内向整流通道，受Na+抑制。K+的吸收和转运主要通过转运蛋白和通道完成，其中Shaker家族基因是植物中研究最多的K+通道。然而，在蔷薇科植物中关于该科的信息却少得多。我们进行了全基因组分析，并确定了蔷薇科的Shaker K+通道基因家族成员。我们从梨（Pyrusxbretschneideri）中克隆并鉴定了一个Shaker K+通道KAT 1。从蔷薇科植物中共鉴定出36个Shaker K+通道基因，根据结构特征和系统发育分析将其分为5个亚组。全基因组和分散复制是蔷薇科Shaker K+通道基因家族扩展的主要动力，纯化选择在Shaker K+通道基因的进化中起着关键作用。β-葡萄糖醛酸酶和qRT-PCR分析表明，PbrKAT 1主要在叶片中表达，尤其是在保卫细胞中。PbrKAT 1在非洲爪蟾卵母细胞中表达时显示出典型的内向整流电流。PbrKAT 1的活性受到外源钠离子的抑制，可能在梨耐盐性的调节中起重要作用。这些结果为研究Shaker钾通道基因家族在植物中的进化、表达和功能提供了有价值的信息。
Main conclusionPbrKAT1, which is inhibited by external Na+ in Xenopus laevis oocytes, is characterized as encoding a typical inward rectifying channel that is mainly expressed in guard cells.AbstractPotassium (K+) is the most abundant cation in plant cells necessary for plant growth and development. The uptake and transport of K+ are mainly completed through transporters and channels, and the Shaker family genes are the most studied K+ channels in plants. However, there is far less information about this family in Rosaceae species. We performed a genome-wide analysis and identified Shaker K+ channel gene family members in Rosaceae. We cloned and characterized a Shaker K+ channel KAT1 from pear (Pyrusxbretschneideri). In total, 36 Shaker K+ channel genes were identified from Rosaceae species and were classified into five subgroups based on structural characteristics and a phylogenetic analysis. Whole-genome and dispersed duplications were the primary forces underlying Shaker K+ channel gene family expansion in Rosaceae, and purifying selection played a key role in the evolution of Shaker K+ channel genes. beta -Glucuronidase and qRT-PCR assays revealed that PbrKAT1 was mainly expressed in leaves, especially in guard cells. PbrKAT1 displayed a typical inward-rectifying current when expressed in Xenopus laevis oocytes. The activity of PbrKAT1 was inhibited by external sodium ions, possibly playing an important role in the regulation of salt tolerance in pear. These results provide valuable information on evolution, expression and functions of the Shaker K+ channel gene family in plants.