Conjugated linoleic acid differentially modulates growth, tissue lipid deposition, and gene expression involved in the lipid metabolism of grass carp
Conjugated linoleic acid differentially modulates growth, tissue lipid deposition, and gene expression involved in the lipid metabolism of grass carp
复制标题
共轭亚油酸差异调节草鱼生长、组织脂质沉积和参与脂质代谢的基因表达
DOI：
10.1016/j.aquaculture.2014.05.008
复制
发表时间：
2014-08-20
期刊：
影响因子：
4.5
通讯作者：
Yang, Yan-ou
中科院分区：
文献类型：
--
作者：
Dong, Gui-Fang;Zou, Qi;Yang, Yan-ou
共轭亚油酸（CLA）已被证明可以减少哺乳动物的体脂和增加瘦肉组织。然而，有关CIA对鱼类组织中脂质含量的影响的数据有限，并且CLA在鱼类中的有益作用的机制尚不清楚。我们推测，饲料CIA可能会诱导草鱼（Ctenopharyngodon idella）组织的降脂作用，和脂肪的减少效果的基因参与脂质代谢的表达调控。通过65 d的生长试验，研究了CIA对草鱼生长、组织脂质沉积和脂质代谢相关基因表达的影响。配制了7种等氮和等脂饮食：0% CIA（对照）; 0.5% CIA（CLA0.5）; 1% CIA（CLA 1）; 1.5% CIA（CLA1.5）; 2% CIA（CLA 2）; 2.5% CIA（CLA 25）;和3% CIA（CLA 3）。结果表明，与对照组相比，只有CLA 3组的摄食率和特定生长率显著降低（P < 0.05）。分别在2.5%~ 3%CIA、1.5%~ 3%CIA和2%~ 3%CIA饲料中观察到肝脏、腹腔脂肪和肌肉中脂质含量的显著降低（P
Conjugated linoleic acid (CLA) has been shown to decrease body fat and increase lean tissue in mammals. However, limited data is available about the effect of CIA on the lipid content in fish tissue, and the mechanisms underlying the beneficial effects of CLA in fish are unknown. We hypothesized that dietary CIA may induce lipid-lowering effects in grass carp (Ctenopharyngodon idella) tissue, and the fat reduction effect was modulated by the expression of genes involved in the lipid metabolism. A 65-day growth trial was conducted to investigate the effect of CIA on the growth, tissue lipid deposition, and gene expression involved in the lipid metabolism of grass carp. Seven isonitrogenous and isolipidic diets were formulated: 0% CIA (control); 0.5% CIA (CLA0.5); 1% CIA (CLA1); 1.5% CIA (CLA1.5); 2% CIA (CLA2); 2.5% CIA (CLA25); and 3% CIA (CLA3). Results showed that only fish fed the CLA3 diet exhibited a significant reduction in feeding rate and specific growth rate than those of fish fed the control diet (P < 0.05). Significant decreases in the lipid content in the liver, intraperitoneal fat, and muscle were observed in fish fed with 2.5% to 3% CIA, 1.5% to 3% CIA, and 2% to 3% CIA diets, respectively (P