A 1H NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects
A 1H NMR-based metabonomic study of urine and plasma samples obtained from healthy human subjects
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DOI：
10.1016/s0731-7085(03)00410-2
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发表时间：
2003-12-04
影响因子：
3.4
通讯作者：
Nash, AFP
中科院分区：
文献类型：
--
作者：
Lenz, EM;Bright, J;Nash, AFP
该研究的目的是评估代谢组学在临床研究中的可行性。对12名健康男性受试者的血浆和尿液样本进行了基于H-1核磁共振（NMR）的代谢组学分析，这些样本分别在两个独立的研究天（间隔14天）进行。受试者喂食标准饮食，并在两天内采集血浆和尿液样本。使用主成分分析（PCA）对尿液和血浆样品的1H NMR谱进行分析，以生成代谢组学数据。在血浆中，受试者和研究时间之间的差异相对较小。在内源性尿液代谢物谱的情况下，受试者之间存在相当大的变异性，但受试者内部的变异性较少。所有受试者的尿液样本均有日变化。这表明在临床研究中收集一致的代谢组学数据是可能的。(C) 2003 Elsevier B.V.版权所有
The aim of the study was to assess the feasibility of metabonomics in clinical studies. A H-1 nuclear magnetic resonance (NMR)-based metabonomic analysis was performed on plasma and urine samples obtained from a group of 12 healthy male subjects on two separate study days 14 days apart. The subjects were fed a standard diet and plasma and urine samples were obtained on both days. The 1H NMR spectra obtained for urine and plasma samples were analysed using principal components analysis (PCA) in order to generate metabonomic data. In plasma there was relatively little variability between subjects and study days. In the case of endogenous urinary metabolite profiles there was considerable inter-subject variability, but less intra-subject variation. In all subjects diurnal variation was seen with urine samples. This suggests the possibility to collect consistent metabonomics data in clinical studies. (C) 2003 Elsevier B.V. All rights reserved.