The DEAD box RNA helicase VBH‐1 is required for germ cell function in C. elegans
The DEAD box RNA helicase VBH‐1 is required for germ cell function in C. elegans
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DOI：
10.1002/dvg.20323
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发表时间：
2007-09
期刊：
影响因子：
1.5
通讯作者：
L. S. Salinas;E. Maldonado;M. Macías-Silva;T. Blackwell;R. Navarro
中科院分区：
文献类型：
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作者：
L. S. Salinas;E. Maldonado;M. Macías-Silva;T. Blackwell;R. Navarro
Vasa和Belle是生殖细胞功能所需的保守的DEAD盒RNA解旋酶。这组蛋白在包括哺乳动物在内的几个物种中的同源物能够补充酵母中的一个突变（DED1），这表明它们的功能是高度保守的。已经提出这些蛋白是mRNA翻译调控所必需的，但它们的具体作用机制尚不清楚。在这里，我们描述了与Belle和Vasa密切相关的秀丽隐杆线虫蛋白VBH‐1的功能。VBH‐1在秀丽隐杆线虫种系中特异性表达，在那里它与秀丽隐杆线虫的种质对应物P颗粒相关。vbh‐1（RNAi）动物由于卵母细胞和精子产生缺陷以及胚胎致死性而比野生型产生更少的后代。我们还发现VBH‐1参与雌雄同体性腺中精子/卵母细胞的转换。我们得出结论，VBH‐1及其同源基因可能在生育和发育中发挥保守的作用。创世纪45:53 - 546,2007。©2007 Wiley‐Liss, Inc。
Vasa and Belle are conserved DEAD box RNA helicases required for germ cell function. Homologs of this group of proteins in several species, including mammals, are able to complement a mutation in yeast (DED1) suggesting that their function is highly conserved. It has been proposed that these proteins are required for mRNA translation regulation, but their specific mechanism of action is still unknown. Here we describe functions of VBH‐1, a C. elegans protein closely related to Belle and Vasa. VBH‐1 is expressed specifically in the C. elegans germline, where it is associated with P granules, the C. elegans germ plasm counterpart. vbh‐1(RNAi) animals produce fewer offspring than wild type because of defects in oocyte and sperm production, and embryonic lethality. We also find that VBH‐1 participates in the sperm/oocyte switch in the hermaphrodite gonad. We conclude that VBH‐1 and its orthologs may perform conserved roles in fertility and development. genesis 45:533–546, 2007. © 2007 Wiley‐Liss, Inc.