Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.
Transplantation of cultured dental pulp stem cells into the skeletal muscles ameliorated diabetic polyneuropathy: therapeutic plausibility of freshly isolated and cryopreserved dental pulp stem cells.
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DOI：
10.1186/s13287-015-0156-4
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发表时间：
2015-09-07
影响因子：
7.5
通讯作者：
Naruse K
中科院分区：
文献类型：
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作者：
Hata M;Omi M;Kobayashi Y;Nakamura N;Tosaki T;Miyabe M;Kojima N;Kubo K;Ozawa S;Maeda H;Tanaka Y;Matsubara T;Naruse K
牙髓干细胞（Dental pulp stem cells，DPSC）是牙髓中的间充质干细胞，由于其可以从正畸拔牙的牙齿中获得，因此被认为是一种潜在的细胞治疗来源。获得的DPSC可以冷冻保存直到需要时，并在需要时解冻和扩增。本研究的目的是评估DPSC移植治疗糖尿病多发性神经病的潜力。将从Sprague-Dawley大鼠的拔牙的牙髓中分离的DPSC在-80 °C冰箱中部分冷冻6个月。注射链脲佐菌素8周后将培养的DPSC移植到一侧后肢骨骼肌中，4周后评价DPSC移植的效果。DPSCs移植可明显改善糖尿病大鼠移植侧受损坐骨神经血流量、坐骨运动/感觉神经传导速度、毛细血管数/肌纤维比和表皮内神经纤维密度。DPSC的冷冻保存并不损害其增殖或分化能力。与新鲜分离的DPSCs相比，冻存DPSCs移植可改善坐骨神经血流量和坐骨神经传导速度。我们证明了DPSC移植治疗糖尿病多发性神经病变的有效性，即使使用冷冻保存的DPSC，这表明DPSC移植可能是治疗糖尿病神经病变的一种有前途的工具。本文的在线版本（doi：10.1186/s13287-015-0156-4）包含补充材料，可供授权用户使用。
Dental pulp stem cells (DPSCs) are mesenchymal stem cells located in dental pulp and are thought to be a potential source for cell therapy since DPSCs can be easily obtained from teeth extracted for orthodontic reasons. Obtained DPSCs can be cryopreserved until necessary and thawed and expanded when needed. The aim of this study is to evaluate the therapeutic potential of DPSC transplantation for diabetic polyneuropathy. DPSCs isolated from the dental pulp of extracted incisors of Sprague–Dawley rats were partly frozen in a −80 °C freezer for 6 months. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles 8 weeks after streptozotocine injection and the effects of DPSC transplantation were evaluated 4 weeks after the transplantation. Transplantation of DPSCs significantly improved the impaired sciatic nerve blood flow, sciatic motor/sensory nerve conduction velocity, capillary number to muscle fiber ratio and intra-epidermal nerve fiber density in the transplanted side of diabetic rats. Cryopreservation of DPSCs did not impair their proliferative or differential ability. The transplantation of cryopreserved DPSCs ameliorated sciatic nerve blood flow and sciatic nerve conduction velocity as well as freshly isolated DPSCs. We demonstrated the effectiveness of DPSC transplantation for diabetic polyneuropathy even when using cryopreserved DPSCs, suggesting that the transplantation of DPSCs could be a promising tool for the treatment of diabetic neuropathy. The online version of this article (doi:10.1186/s13287-015-0156-4) contains supplementary material, which is available to authorized users.