LIGHT MICROSCOPIC AUTORADIOGRAPHY FOR STUDY OF EARLY CHANGES IN THE DISTRIBUTION OF WATER-SOLUBLE MATERIALS
LIGHT MICROSCOPIC AUTORADIOGRAPHY FOR STUDY OF EARLY CHANGES IN THE DISTRIBUTION OF WATER-SOLUBLE MATERIALS
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DOI：
10.1177/38.12.2254645
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发表时间：
1990-12-01
影响因子：
3.2
通讯作者：
KAWAMOTO, T
中科院分区：
文献类型：
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作者：
KAWAMOTO, T
研究了一种利用放射自显影技术来研究水溶性物质分布的早期变化和所涉及的化学成像的方法。将放射性氯化钙(45Ca)注入大鼠的髂静脉。注射后10秒，大鼠被冰冻在正己烷(-90度)中。c)。将冷冻的大鼠包埋在5%的羧甲基纤维素钠中，并将其封堵在冷却液中。将一张涂有合成橡胶胶的塑料胶带固定在修剪后的块表面，用一次性切割刀切割2-10微米厚的全身部分。选定的切片被冷冻干燥，然后用干燥的放射自显影乳胶片覆盖，厚度约为1微米。放射自显影清楚地显示了放射性钙在长骨钙化区的分布。选择用来评估化学伪影的样本，当这些组织保持在23度时，大多数组织上都显示出阳性和阴性的化学图谱。C，尽管当样品保持在-20℃时，这两种化学效应都显著降低。C、几种组织中的细胞仍呈现阳性和阴性化学特征。该技术可用于研究任何大小适合全身冰冻切片的动物。
An approach using autoradiography for the study of early changes in the distribution of water-soluble materials and the chemography involved was investigated. Radioactive calcium chloride (45Ca) was injected into the iliac vein of rat. Ten seconds after the injection the rat was frozen in hexane (-90.degree. C). The frozen rat was embedded in 5% sodium carboxymethyl cellulose and blocked in the coolant. A sheet of plastic tape coated with a synthetic rubber glue was fastened to the trimmed block surface, and whole-body sections 2-10 .mu.m thick were cut with a disposable microtome knife. Selected sections were freeze-dried and then covered with a dried autoradiographic emulsion film about 1 .mu.m thick. The autoradiograph clearly showed the distribution of radioactive calcium in the calcification zone of long bones. The samples chosen to assess chemographic artifacts showed positive and negative chemographies on most of the tissues when these were kept at 23.degree. C, and although both chemographic effects were significantly reduced when the samples were kept at -20.degree. C, cells in several tissues still exhibited positive and negative chemographies. The technique can be used for the study of any animal whose size is suitable for whole-body freeze-sectioning.