READING FRAME SWITCH CAUSED BY BASE-PAIR FORMATION BETWEEN THE 3' END OF 16S RIBOSOMAL-RNA AND THE MESSENGER-RNA DURING ELONGATION OF PROTEIN-SYNTHESIS IN ESCHERICHIA-COLI
READING FRAME SWITCH CAUSED BY BASE-PAIR FORMATION BETWEEN THE 3' END OF 16S RIBOSOMAL-RNA AND THE MESSENGER-RNA DURING ELONGATION OF PROTEIN-SYNTHESIS IN ESCHERICHIA-COLI
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DOI：
10.1002/j.1460-2075.1988.tb02969.x
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发表时间：
1988-05-01
期刊：
影响因子：
11.4
通讯作者：
GESTELAND, RF
中科院分区：
文献类型：
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作者：
WEISS, RB;DUNN, DM;GESTELAND, RF
在大肠杆菌蛋白质合成的延伸阶段，Watson-Crick 碱基配对发生在 16S rRNA 3'' 末端附近的 mRNA 和核苷酸之间。这种碱基配对类似于在核糖体结合位点的 Shine 和 Dalgarno (S-D) 核苷酸及其在 16S rRNA 1540-1535 区域中的互补体之间形成的蛋白质合成起始过程中形成的 mRNA-rRNA 相互作用，延伸过程中 mRNA-rRNA 杂交体的形成已被假设来解释有效核糖体移码对 mRNA 上精确间隔 5'' 的 S-D 核苷酸的依赖性来自移码站点。在这里，我们表明，通过单核苷酸替换（在移位所需的 S-D 序列中或在 16S rRNA 的核苷酸 1538 中）破坏假定的碱基对，会减少移位量，并且通过恢复互补碱基配对可以纠正这种缺陷。该结果表明 16S rRNA 的 3'' 末端在延伸过程中扫描非常靠近解码位点的 mRNA。
Watson-Crick base pairing is shown to occur between the mRNA and nucleotides near the 3'' end of 16S rRNA during the elongation phase of protein synthesis in Escherichia coli. This base-pairing is similar to the mRNA-rRNA interaction formed during initiation of protein synthesis between the Shine and Dalgarno (S-D) nucleotides of ribosome binding sites and their complements in the 1540-1535 region of 16S rRNA, mRNA-rRNA hybrid formation during elongation had been postulated to explain the dependence of an efficient ribosomal frameshift on S-D nucleotides precisely spaced 5'' on the mRNA from the frameshift site. Here we show that disruption of the postulated base pairs by single nucleotide substitutions, either in the S-D sequence required for shifting or in nucleotide 1538 of 16S rRNA, decrease the amount of shifting, and that this defect is corrected by restoring complementary base pairing. This result implies that the 3'' end of 16S rRNA scans the mRNA very close to the decoding sites during elongation.