Altered blood-brain barrier permeability and its effect on the distribution of Evans blue and sodium fluorescein in the rat brain applied by intracarotid injection
Altered blood-brain barrier permeability and its effect on the distribution of Evans blue and sodium fluorescein in the rat brain applied by intracarotid injection
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DOI：
10.33549/physiolres.930289
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发表时间：
2003-01-01
影响因子：
2.1
通讯作者：
Pokorny, J
中科院分区：
文献类型：
--
作者：
Kozler, P;Pokorny, J
根据伊文思蓝(EB)和荧光素钠(NaF1)在大鼠脑内的分布，研究颈动脉注射对血脑通透性的影响。根据应用染料时血脑屏障(BBB)的状态，将18只动物分为6组。在前两组中，血脑屏障是完整的，在第3组和第4组中，屏障在应用染料之前已经被渗透开放，而在第5组和第6组中，在给药前通过高水合作用诱导了细胞水肿。用荧光显微镜研究了染料在胞内和胞外的分布。因此，组织学图片代表了在应用染料之前血脑屏障通透性变化的形态相关性。
The aim was to study the blood-brain permeability according to the distribution in the rat brain of Evans blue (EB) and sodium fluorescein (NaFl) administered by an intracarotid injection. Eighteen animals were divided into six groups according to the state of the blood-brain barrier (BBB) at the moment when the dyes were being applied. In the first two groups, the BBB was intact, in groups 3 and 4 the barrier had been opened osmotically prior to the application of the dyes, and in groups 5 and 6 a cellular edema was induced by hyperhydration before administration of the dyes. The intracellular and extracellular distribution of the dyes was studied by fluorescence microscopy. The histological picture thus represented the morphological correlate of the way BBB permeability had been changed before the application of the dyes.