Agatharesinol biosynthesis-related changes of ray parenchyma in sapwood sticks of Cryptomeria japonica during cell death
Agatharesinol biosynthesis-related changes of ray parenchyma in sapwood sticks of Cryptomeria japonica during cell death
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DOI：
10.1007/s00425-016-2473-y
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发表时间：
2016-05-01
期刊：
影响因子：
4.3
通讯作者：
Funada, Ryo
中科院分区：
文献类型：
--
作者：
Nakaba, Satoshi;Arakawa, Izumi;Funada, Ryo
这项工作证明了次生代谢产物，agatharesinol的生物合成之间的关系，和细胞学的变化，发生在射线薄壁组织中的细胞死亡过程中柳杉边材棍棒在湿度调节conditions.To表征射线薄壁组织细胞的死亡，伴随着次生代谢产物的生物合成，我们研究了细胞死亡柳杉边材棍棒在湿度调节条件下。我们监测射线薄壁细胞的功能，如生活力，核和液泡的形态，和淀粉粒的量。此外，我们分析了水平agatharesinol，心材降木脂素，气相色谱-质谱法在相同的边材棒。淀粉粒的数量和阿加西酚的水平同时发生显着变化。因此，沉香树脂酚的生物合成似乎起源于淀粉的分解。此外，我们在细胞死亡的最后阶段观察到射线薄壁细胞中液泡的扩张先于其他细胞学变化。在我们的实验系统中，我们能够遵循细胞死亡的过程，并证明细胞学变化和射线薄壁细胞死亡过程中次生代谢产物的生物合成之间的关系。
The work demonstrates a relationship between the biosynthesis of the secondary metabolite, agatharesinol, and cytological changes that occur in ray parenchyma during cell death in sapwood sticks of Cryptomeria japonica under humidity-regulated conditions.To characterize the death of ray parenchyma cells that accompanies the biosynthesis of secondary metabolites, we examined cell death in sapwood sticks of Cryptomeria japonica under humidity-regulated conditions. We monitored features of ray parenchyma cells, such as viability, the morphology of nuclei and vacuoles, and the amount of starch grains. In addition, we analyzed levels of agatharesinol, a heartwood norlignan, by gas chromatography-mass spectrometry in the same sapwood sticks. Dramatic changes in the amount of starch grains and in the level of agatharesinol occurred simultaneously. Therefore, the biosynthesis of agatharesinol appeared to originate from the breakdown of starch. Furthermore, we observed the expansion of vacuoles in ray parenchyma cells prior to other cytological changes at the final stage of cell death. In our experimental system, we were able to follow the process of cell death and to demonstrate relationships between cytological changes and the biosynthesis of a secondary metabolite during the death of ray parenchyma cells.