Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: Implications for axial patterning
Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: Implications for axial patterning
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DOI：
10.1006/dbio.2002.0657
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发表时间：
2002-06-15
影响因子：
2.7
通讯作者：
Layton, JE
中科院分区：
文献类型：
--
作者：
Lieschke, GJ;Oates, AC;Layton, JE
哺乳动物转录因子SPI-1（同义词：SPI1， PU.1或Sfpi1）在髓细胞发育中起关键作用。为了研究斑马鱼胚胎中的早期髓系承诺，我们从斑马鱼中分离出一个基于同源性和系统发育考虑的SPI-1同源基因。斑马鱼spi1 （pu1）基因在受精后12 h在吻侧外侧板中胚层（LPM）中首次表达，解剖分离于尾侧外侧板中胚层的红系发育。命运定位追踪了吻侧LPM细胞从初始spi1表达区域到髓系命运。在无血突变体c10che中Spi1的表达缺失，但在突变体spadetail中，尽管其红细胞生成完全失败，但仍保留了吻侧Spi1的表达和髓系发育。在对BMP-4缺失型突变体和蜗牛室突变体以及chorordino缺失型突变体中过表达BMP-4或chordin的胚胎的研究中，进一步探讨了髓系和红细胞发育的分离。这些研究表明，在斑马鱼中，spi1标志着一个吻侧LPM细胞群体致力于髓系命运，在解剖上与尾侧LPM的红系命运分离，并且在发育上独立于红系命运。这两种造血谱系的完全解剖和发育分离增加了对脊椎动物造血发育的理解的有趣复杂性，并对轴向模式的调节机制提出了重要的影响。(C) 2002 Elsevier Science （USA）。
The mammalian transcription factor SPI-1 (synonyms: SPI1, PU.1, or Sfpi1) plays a critical role in myeloid development. To examine early myeloid commitment in the zebrafish embryo, we isolated a gene from zebrafish that is a SPI-1 orthologue on the basis of homology and phylogenetic considerations. The zebrafish spi1 (pu1) gene was first expressed at 12 h postfertilization in rostral lateral plate mesoderm (LPM), anatomically isolated from erythroid development in caudal lateral plate mesoderm. Fate-mapping traced rostral LPM cells from the region of initial spi1 expression to a myeloid fate. spi1 expression was lost in the bloodless mutant c1oche, but rostral spi1 expression and myeloid development were preserved in the mutant spadetail, despite its complete erythropoietic failure. This dissociation of myeloid and erythroid development was further explored in studies of embryos overexpressing BMP-4, or chordin, in bmp-deficient swir1 and snailhouse mutants, and chordin-deficient chordino mutants. These studies demonstrate that, in zebrafish, spi1 marks a rostral population of LPM cells committed to a myeloid fate anatomically separated from and developmentally independent of erythroid commitment in the caudal LPM. Such complete anatomical and developmental dissociation of two hematopoietic lineages adds an interesting complexity to the understanding of vertebrate heniatopoictic development and presents significant implications for the mechanisms regulating axial patterning. (C) 2002 Elsevier Science (USA).