Rrs1p, a ribosomal protein L11-binding protein, is required for nuclear export of the 60S pre-ribosomal subunit in Saccharomyces cerevisiae
Rrs1p, a ribosomal protein L11-binding protein, is required for nuclear export of the 60S pre-ribosomal subunit in Saccharomyces cerevisiae
复制标题
DOI：
10.1016/j.febslet.2004.03.087
复制
发表时间：
2004-05-01
期刊：
影响因子：
3.5
通讯作者：
Mizuta, K
中科院分区：
文献类型：
--
作者：
Miyoshi, K;Shirai, C;Mizuta, K
Rrs1p是酿酒酵母核糖体蛋白L11结合蛋白。通过随机聚合酶链式反应诱变获得了温度敏感型rrs1突变体。[H-3]蛋氨酸脉冲追逐分析表明，rrs1突变导致25S rRNA成熟缺陷。核糖体蛋白L25增强的绿色荧光蛋白是60S核糖体亚基的报告蛋白，当rrs1突变体转移到限制性温度时，它集中在细胞核中，并在核仁中富集。这些结果表明，从大亚基成熟的早期到很晚阶段，Rrs1p一直停留在60S前的颗粒上，是将60S亚基从核仁输出到细胞质所必需的。(C)2004年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Rrs1p is a ribosomal protein L11-binding protein in Saccharomyces cerevisiae. We have obtained temperature-sensitive rrs1 mutants by random PCR mutagenesis. [H-3]Methionine pulse-chase analysis reveals that the rrs1 mutations cause a defect in maturation of 25S rRNA. Ribosomal protein L25-enhanced green fluorescent protein, a reporter of the 60S ribosomal subunit, concentrates in the nucleus with enrichment in the nucleolus when the rrs1 mutants are shifted to the restrictive temperature. These results suggest that Rrs1p stays on the pre-60S particle from the early stage to very late stage of the large-subunit maturation and is required for export of 60S subunits from the nucleolus to the cytoplasm. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.