Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope
Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope
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DOI：
10.1002/mp.16599
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发表时间：
2023-07-11
期刊：
影响因子：
3.8
通讯作者：
Olivo，Alessandro
中科院分区：
文献类型：
--
作者：
Esposito，Michela;Astolfo，Alberto;Olivo，Alessandro
背景软骨的显微成像是研究和开发骨关节炎治疗方法的关键工具。当需要细胞和亚细胞分辨率时，组织学仍然是金标准方法，尽管受到缺乏体积信息以及处理伪影的限制。具有亚细胞分辨率的软骨成像仅在同步加速器environment.PurposeTo提供基于实验室的X射线相衬显微镜分辨软骨样品中的亚细胞特征的能力的概念验证。MethodsThis work is based on a laboratory-based X射线显微镜using intensity-modulation masks.由掩模孔径产生的光束的结构性质允许检索三个对比度通道，即透射、折射和暗场，分辨率仅取决于掩模孔径宽度。Anex vivoequine软骨样品成像与X射线显微镜和结果进行了验证与同步辐射断层扫描和histologics.ResultsIndividual软骨细胞，即负责软骨形成的细胞，可以检测到与实验室为基础的显微镜。三个检索的对比通道的互补性允许检测的亚细胞特征在软骨cytos.ConclusionsWe提供了第一个证明概念的成像软骨组织与亚细胞分辨率使用基于实验室的X射线显微镜。
BackgroundMicroscopic imaging of cartilage is a key tool for the study and development of treatments for osteoarthritis. When cellular and sub‐cellular resolution is required, histology remains the gold standard approach, albeit limited by the lack of volumetric information as well as by processing artifacts. Cartilage imaging with the sub‐cellular resolution has only been demonstrated in the synchrotron environment.PurposeTo provide a proof‐of‐concept demonstration of the capability of a laboratory‐based x‐ray phase‐contrast microscope to resolve sub‐cellular features in a cartilage sample.MethodsThis work is based on a laboratory‐based x‐ray microscope using intensity‐modulation masks. The structured nature of the beam, resulting from the mask apertures, allows the retrieval of three contrast channels, namely, transmission, refraction and dark‐field, with resolution depending only on the mask aperture width. Anex vivoequine cartilage sample was imaged with the x‐ray microscope and results were validated with synchrotron tomography and histology.ResultsIndividual chondrocytes, that is, cells responsible for cartilage formation, could be detected with the laboratory‐based microscope. The complementarity of the three retrieved contrast channels allowed the detection of sub‐cellular features in the chondrocytes.ConclusionsWe provide the first proof‐of‐concept of imaging cartilage tissue with sub‐cellular resolution using a laboratory‐based x‐ray microscope.