Identification of natural antisense transcripts involved in human colorectal cancer development
Identification of natural antisense transcripts involved in human colorectal cancer development
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DOI：
10.3892/ijo_00000794
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发表时间：
2010-12-01
影响因子：
5.2
通讯作者：
Ohkohchi, Nobuhiro
中科院分区：
文献类型：
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作者：
Kohno, Keisuke;Chiba, Mitsuru;Ohkohchi, Nobuhiro
天然反义转录本 (NAT) 构成一类非编码 RNA，已成为基因表达的重要调节因子。然而，迄今为止 NAT 参与结直肠癌 (CRC) 发展的报道尚未见报道。本研究对人类 CRC 中 NAT 的上调和下调是否可能参与 CRC 发展进行了研究。对从手术切除样本中的 51 个 CRC 组织、9 个相应的非癌组织和 19 个肝转移组织中分离出的总 RNA 进行了研究。使用包含约 21,000 个基因的人类有义/反义探针的定制微阵列进行表达分析比较 CRC 组织与非癌组织，我们鉴定了 415 个 NAT 在 CRC 和非癌组织中显着差异表达（p4 0 或
Natural antisense transcripts (NATs) constitute a class of non-coding RNAs that have emerged as important regulators of gene expression However involvement of NATs in colorectal cancer (CRC) development has not been reported to date In the present study, the up- and downregulation of NATs were investigated in human CRC for their possible involvement in CRC development Total RNAs isolated from 51 CRC tissues, 9 corresponding non-cancerous tissues and 19 liver metastatic tissues from surgically resected samples were subjected to expression analysis using a custom-microarray containing human sense/ antisense probes for ca 21,000 genes Comparing CRC tissues with non-cancerous tissues, we identified 415 NATs differentially expressed in CRC and non-cancerous tissues to a significant degree (p4 0 or