Failed Escape: Solid Surfaces Prevent Tumbling of Escherichia coli
Failed Escape: Solid Surfaces Prevent Tumbling of Escherichia coli
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DOI：
10.1103/physrevlett.113.068103
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发表时间：
2014-08-07
影响因子：
8.6
通讯作者：
Sheng, Jian
中科院分区：
文献类型：
--
作者：
Molaei, Mehdi;Barry, Michael;Sheng, Jian
了解细菌如何接近表面对于包括生物膜形成、细菌分散和致病性感染在内的广泛微生物过程至关重要。我们使用数字全息显微镜捕捉了大量（bbb10(3)）的三维大肠杆菌的轨迹近和远的表面。我们发现，在距离地表20米的范围内，翻滚被抑制了50%，并且重新定向主要局限于表面平行方向，防止了细菌从近地表区域逃逸。水动力模型表明，翻滚抑制可能是由于表面诱导的水动力减少，导致鞭毛解束，导致翻滚。这些发现表明，翻滚并不能提供一种有效的方法来逃避表面附近的陷阱。
Understanding how bacteria move close to surfaces is crucial for a broad range of microbial processes including biofilm formation, bacterial dispersion, and pathogenic infections. We used digital holographic microscopy to capture a large number (>10(3)) of three-dimensional Escherichia coli trajectories near and far from a surface. We found that within 20 m from a surface tumbles are suppressed by 50% and reorientations are largely confined to surface-parallel directions, preventing escape of bacteria from the near-surface region. A hydrodynamic model indicates that the tumble suppression is likely due to a surface-induced reduction in the hydrodynamic force responsible for the flagellar unbundling that causes tumbling. These findings imply that tumbling does not provide an effective means to escape trapping near surfaces.