Plasma sphingosine-1-phosphate measurement in healthy subjects: close correlation with red blood cell parameters
Plasma sphingosine-1-phosphate measurement in healthy subjects: close correlation with red blood cell parameters
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DOI：
10.1258/acb.2007.007189
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发表时间：
2008-07-01
影响因子：
2.2
通讯作者：
Yatomi, Yutaka
中科院分区：
文献类型：
--
作者：
Ohkawa, Ryunosuke;Nakamura, Kazuhiro;Yatomi, Yutaka
背景：由于1-磷酸鞘氨醇(sph-1-P)通过与特定的细胞表面受体相互作用而发挥重要的细胞外介质作用，特别是在血管生物学和免疫/血液学领域，测定其血药浓度具有重要的临床意义。因此，我们试图建立一种用于临床实验室的血浆sph-1-P浓度的测定方法。方法：将sph-1-P与邻苯二甲醛两步提取脂后，用高效液相色谱分离得到荧光衍生物。以C-17-SPH-1-P为内标，取代以往用于相同目的的二氢鞘氨醇-1-磷酸，实际在血浆中检测到。结果：我们的血浆样品制备和SPH-1-P的测定方法是令人满意的，用于临床实验室检测。男性血浆SPH-1-P浓度(413.1+/-52.0nmol/L；均值+/-SD)显著高于女性(352.4+/-39.7nmol/L)。出乎意料的是，血浆Sph-1-P浓度与红细胞(RBC)相关参数呈正相关，而与血小板相关参数无关。结论：本研究证实了将血浆Sph-1-P检测引入临床的可能性，并提示红细胞可能参与了血浆Sph-1-P浓度的调节。
Background: Since sphingosine-1-phosphate (Sph-1-P) plays an important role as an extracellular mediator through interaction with specific cell surface receptors, especially in the area of vascular biology and immunology/haematology, determination of its plasma concentration may become important from the clinical viewpoint. Thus, we attempted to develop a method of measuring the plasma Sph-1-P concentration for use in the clinical laboratory setting.Methods: After two-step lipid extraction, Sph-1-P was coupled with o-phthaldialdehyde, and the resultant fluorescent derivative was separated by high-performance liquid chromatography. C-17-Sph-1-P was used as the internal standard, instead of dihydrosphingosine-1-phosphate, which had been used previously for the same purpose but was actually detected in plasma.Results: Our procedures for preparing the plasma samples and assay Sph-1-P were found to be satisfactory for clinical laboratory testing. The plasma Sph-1-P concentrations were significantly higher in men (413.1 +/- 52.0 nmol/L; mean +/- SD) than in women (352.4 +/- 39.7 nmol/L). Unexpectedly, strong positive correlations were found between the plasma Sph-1-P concentration and red blood cell (RBC)-related parameters, rather than platelet-related parameters.Conclusions: Our present study confirmed the possibility of the clinical introduction of plasma Sph-1-P measurement, and in addition, suggested that RBCs may be involved in the regulation of plasma Sph-1-P concentrations.