Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus
Feline coronavirus type II strains 79-1683 and 79-1146 originate from a double recombination between feline coronavirus type I and canine coronavirus
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DOI：
10.1128/jvi.72.5.4508-4514.1998
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发表时间：
1998-05-01
影响因子：
5.4
通讯作者：
de Groot, RJ
中科院分区：
文献类型：
--
作者：
Herrewegh, AAPM;Smeenk, I;de Groot, RJ
最近的证据表明，II型猫冠状病毒（FCoV）菌株79-1146和79-1683是由FCoV I型和犬冠状病毒（CCV）之间的同源RNA重组事件引起的。在这两种情况下，模板转换显然发生在S和M基因之间，产生编码CCV样S蛋白和I型FCoV的M、N、7a和7 b蛋白的重组病毒（K. Motowaka，T. Hohdatsu，H. Hashimoto和H. Koyama，微生物。Immunol.40：425-433，1996; H. Vennema，A.波兰，K.弗洛伊德霍金斯和N. C. Pedersen，Feline Pract.23：40-44，1995）。在本研究中，我们已经寻找了额外的FCoV-CCV重组位点。选择pol基因中的四个区域用于II型FCoV毒株79-1683和79-1146、I型FCoV毒株TN 406和UCD 1、CCV毒株K378和TGEV毒株Purdue的比较序列分析。我们的数据表明，II型FCoV是由双重组事件引起的：在菌株79-1683的ORF 1ab移码区和菌株79-1146的ORF 1b的5'半中定位了额外的交换位点。
Recent evidence suggests that the type II feline coronavirus (FCoV) strains 79-1146 and 79-1683 have arisen from a homologous RNA recombination event between FCoV type I and canine coronavirus (CCV). In both cases, the template switch apparently took place between the S and M genes, giving rise to recombinant viruses which encode a CCV-like S protein and the M, N, 7a, and 7b proteins of FCoV type I (K. Motowaka, T. Hohdatsu, H. Hashimoto, and H. Koyama, Microbiol. Immunol. 40:425-433, 1996; H. Vennema, A. Poland, K. Floyd Hawkins, and N. C. Pedersen, Feline Pract. 23:40-44, 1995). In the present study, we have looked for additional FCoV-CCV recombination sites. Four regions in the pol gene were selected for comparative sequence analysis of the type II FCoV strains 79-1683 and 79-1146, the type I FCoV strains TN406 and UCD1, the CCV strain K378, and the TGEV strain Purdue. Our data show that the type II FCoVs have arisen from double recombination events: additional crossover sites were mapped in the ORF1ab frameshifting region of strain 79-1683 and in the 5' half of ORF1b of strain 79-1146.