Correlation between physicochemical characteristics of lignin deposited on autohydrolyzed wood chips and their cellulase enzymatic hydrolysis.
Correlation between physicochemical characteristics of lignin deposited on autohydrolyzed wood chips and their cellulase enzymatic hydrolysis.
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DOI：
10.1016/j.biortech.2022.126941
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发表时间：
2022-03
影响因子：
11.4
通讯作者：
Wenchao Jia;Haiqiang Shi;X. Sheng;Yanzhu Guo;P. Fatehi;Meihong Niu
中科院分区：
文献类型：
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作者：
Wenchao Jia;Haiqiang Shi;X. Sheng;Yanzhu Guo;P. Fatehi;Meihong Niu
酶水解是从生物质产生生物燃料的方法，并且自动水解是在酶水解之前对生物质进行预处理的常用方法。本研究的主要目的是确定在生物质的酶水解的自水解过程中产生的木质素的作用。HSQC和~（31）P NMR分析表明，随着水解强度的增加，木质素的β-O-4减少，β-5、β-β和S/G比增加，木质素的缩合和非缩合酚羟基增加。有趣的是，纤维素酶吸附更多的木质素，具有更多的酚含量，它与木质素的缔合降低了其水解纤维素微晶的活性。酶解产率与自水解过程中产生的木质素的缩合S-OH（r2= 0.978）和G-OH（r2= 0.961）呈强负相关。
Enzymatic hydrolysis is a method to generate biofuel from biomass, and autohydrolysis is a popular method to pretreat biomass prior to enzymatic hydrolysis. The primary aim of the present study was to determine the role of lignin produced in the autohydrolysis process on the enzymatic hydrolysis of biomass. The HSQC and31P NMR analyses confirmed that β-O-4 of lignin was reduced, while β-5, β-β, and S/G-ratio of lignin were increased with intensifying the hydrolysis intensity. The increase in the hydrolysis intensity significantly enhanced the condensed and non-condensed phenolic OH group of lignin. Interestingly, the cellulase enzyme adsorbed more on lignin that had more phenolic content, and its association with lignin reduced its activity for hydrolyzing cellulose microcrystals. Strong negative correlations were observed between the enzymatic hydrolysis yield and the condensed S-OH (r2= 0.978) and G-OH (r2= 0.961) of lignin generated in the autohydrolysis process.